Cited 30 times in
HSP90beta interacts with Rac1 to activate NADPH oxidase in Helicobacter pylori-infected gastric epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.date.accessioned | 2015-04-23T16:50:04Z | - |
dc.date.available | 2015-04-23T16:50:04Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1357-2725 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101316 | - |
dc.description.abstract | Reactive oxygen species (ROS) are involved in the pathogenesis of Helicobacter pylori (H. pylori)-induced gastric disorders including inflammation. NADPH oxidase has been considered as a major source of ROS in phagocytic and non-phagocytic cells. Small G protein Rac1 is one components of NADPH oxidase complex. Heat shock protein 90 kDa (HSP90) modulates cytokine production in H. pylori-infected gastric epithelial cells. The present study aims to investigate the relation of HSP90beta and Rac1 on the activation of NADPH oxidase in H. pylori-infected gastric epithelial cells. As a result, H. pylori-induced translocation of HSP90beta from the cytosol to the membrane and activated Rac1 in gastric epithelial AGS cells. HSP90beta physically interacted with Rac1, which resulted in the activation of NADPH oxidase in H. pylori-infected AGS cells. Down-regulation of HSP90beta by transfection of HSP90beta siRNA suppressed the activation of Rac1, activity of NADPH oxidase and the production of H(2)O(2) in H. pylori-infected AGS cells. In conclusion, H. pylori induces the translocation of HSP90beta from the cytosol to the membrane and interaction of HSP90beta and Rac1, which leads to the activation of NADPH oxidase and production of ROS in gastric epithelial cells. HSP90beta may be the target molecule for treatment of H. pylori-induced gastric injury. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1455~1461 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cytosol/metabolism | - |
dc.subject.MESH | Epithelial Cells/metabolism* | - |
dc.subject.MESH | Epithelial Cells/microbiology* | - |
dc.subject.MESH | Gastric Mucosa/cytology | - |
dc.subject.MESH | HSP90 Heat-Shock Proteins/genetics | - |
dc.subject.MESH | HSP90 Heat-Shock Proteins/metabolism* | - |
dc.subject.MESH | Helicobacter Infections/microbiology | - |
dc.subject.MESH | Helicobacter Infections/physiopathology* | - |
dc.subject.MESH | Helicobacter pylori/physiology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoprecipitation | - |
dc.subject.MESH | NADPH Oxidases/genetics | - |
dc.subject.MESH | NADPH Oxidases/metabolism* | - |
dc.subject.MESH | Protein Transport | - |
dc.subject.MESH | RNA, Small Interfering | - |
dc.subject.MESH | rac1 GTP-Binding Protein/genetics | - |
dc.subject.MESH | rac1 GTP-Binding Protein/metabolism* | - |
dc.title | HSP90beta interacts with Rac1 to activate NADPH oxidase in Helicobacter pylori-infected gastric epithelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Soon Ok Cho | - |
dc.contributor.googleauthor | Joo Weon Lim | - |
dc.contributor.googleauthor | Jong-Ho Jun | - |
dc.contributor.googleauthor | Kyung Hwan Kim | - |
dc.contributor.googleauthor | Hyeyoung Kim | - |
dc.identifier.doi | 10.1016/j.biocel.2010.04.015 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00311 | - |
dc.relation.journalcode | J01089 | - |
dc.identifier.eissn | 1878-5875 | - |
dc.identifier.pmid | 20451655 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1357272510001664 | - |
dc.subject.keyword | Helicobacter pylori | - |
dc.subject.keyword | HSP90β | - |
dc.subject.keyword | Rac1 | - |
dc.subject.keyword | NADPH oxidase | - |
dc.subject.keyword | AGS cells | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.citation.volume | 42 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1455 | - |
dc.citation.endPage | 1461 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Vol.42(9) : 1455-1461, 2010 | - |
dc.identifier.rimsid | 49433 | - |
dc.type.rims | ART | - |
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