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Chitinase induce the release of IL-8 in human airway epithelial cells, via Ca2+-dependent PKC and ERK pathways

Authors
 J. Y. Hong  ;  K. E. Lee  ;  K. W. Kim  ;  M. H. Sohn  ;  K.-E. Kim 
Citation
 SCANDINAVIAN JOURNAL OF IMMUNOLOGY, Vol.72(1) : 15-21, 2010 
Journal Title
 SCANDINAVIAN JOURNAL OF IMMUNOLOGY 
ISSN
 0300-9475 
Issue Date
2010
MeSH
Blotting, Western ; Butadienes/pharmacology ; Caffeic Acids/pharmacology ; Carbazoles/pharmacology ; Cell Line, Tumor ; Chitinases/immunology* ; Enzyme-Linked Immunosorbent Assay ; Epithelial Cells ; Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors ; Extracellular Signal-Regulated MAP Kinases/immunology* ; Humans ; Indoles/pharmacology ; Interleukin-8/genetics ; Interleukin-8/immunology* ; NF-kappa B/antagonists & inhibitors ; NF-kappa B/immunology ; Naphthalenes/pharmacology ; Nitriles/pharmacology ; Phenylethyl Alcohol/analogs & derivatives ; Phenylethyl Alcohol/pharmacology ; Protein Kinase C/antagonists & inhibitors ; Protein Kinase C/immunology* ; Protein Kinase Inhibitors/pharmacology ; RNA, Messenger/chemistry ; RNA, Messenger/genetics ; Respiratory Mucosa/enzymology ; Respiratory Mucosa/immunology* ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction
Abstract
Chitinases are produced in significant quantities by hosts defending against infections with chitin-containing organisms. However, little is known about the immune response of exogenous chitinase in human epithelial cells. IL-8 has been suggested to have a role in the pathogenesis of the allergenic inflammation of bronchial asthma. We examined whether Streptomyces griseus (S. griseus) chitinase-induced IL-8 on airway epithelium and identified the involvement of intracellular signalling pathways. H292 cells were treated with S. griseus chitinase with different concentrations and times. The IL-8 levels were determined by specific human IL-8 enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase polymerase chain reaction. Using a series of pharmacological inhibitors, we examined the upstream signalling pathway responsible for IL-8 expression in response to S. griseus chitinase. Cells exposed to S. griseus chitinase showed higher level of IL-8 protein production and mRNA expression. Cells stimulated by S. griseus chitinase resulted in the activation of protein kinase C (PKC), extracellular signal-regulated kinase (ERK) and nuclear factor kappa-B (NF-kB) pathways. Inhibitors of Ca(2+)-dependent PKC (Ro-31-8220, calphostin C and Go6976) significantly abolished chitinase-induced expression of IL-8. However, Ca(2+)-independent PKC inhibitor (rottlerin) did not inhibit IL-8 expression. Through ERK inhibitor (U0126) and NF-kB inhibitor (caffeine acid phenethyl ester) treatment, it was proven that ERK and NF-kB regulated chitinase-induced IL-8 expression. We concluded that S. griseus chitinase-induced IL-8 expression was regulated by the activation of Ca(2+/-)-dependent PKC, ERK and NF-kB in human airway epithelial cells
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2010.02404.x/abstract
DOI
10.1111/j.1365-3083.2010.02404.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Won(김경원) ORCID logo https://orcid.org/0000-0003-4529-6135
Kim, Kyu Earn(김규언)
Sohn, Myung Hyun(손명현) ORCID logo https://orcid.org/0000-0002-2478-487X
Hong, Jung Yeon(홍정연) ORCID logo https://orcid.org/0000-0003-0406-9956
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101268
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