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아텔로콜라겐 지지체, 유전자 치료, 배양된 수핵세포를 이용한 조직 공학적 추간판의 재생
DC Field | Value | Language |
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dc.contributor.author | 권언혜 | - |
dc.contributor.author | 김보람 | - |
dc.contributor.author | 김학선 | - |
dc.contributor.author | 김향 | - |
dc.contributor.author | 남미란 | - |
dc.contributor.author | 문성환 | - |
dc.contributor.author | 이광일 | - |
dc.contributor.author | 이환모 | - |
dc.date.accessioned | 2015-04-23T16:47:45Z | - |
dc.date.available | 2015-04-23T16:47:45Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1229-5701 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101241 | - |
dc.description.abstract | STUDY DESIGN: This is an in-vitro experiment using rabbit intervertebral disc (IVD) cells and growth factors. OBJECTIVES: We wanted to determine the effect of types I, and II atelocollagen and growth factor gene therapy for matrix regeneration of rabbit IVD cells. SUMMARY OF THE LITERATURE REVIEW: Adenovirus-medicated growth factor gene therapy is efficient for matrix regeneration of the IVD. Atellocollagen has provided a favorable environment for matrix synthesis. However, a combined approach using gene and cell therapy in an atelocollagen scaffold has not yet been attempted. MATERIALS AND METHODS: Rabbit IVD cells were transduced with Ad/TGF-beta1 and Ad/BMP-2. The cells were then implanted to the atelocollagen scaffold. The [methyl-3H]thymidine incorporation for DNA synthesis and the [35S]sulfur incorporation for proteoglycan synthesis were measured. RT-PCR was performed for assessing the aggrecan, collagen type I, collagen type II and osteocalcin mRNA expressions. RESULTS: The rabbit IVD cells with Ad/TGF-beta1 and that were cultured in type I atelocollagen showed a 130% increase in new proteoglycan synthesis, while the rabbit IVD cells with Ad/TGF-beta1 and that were cultured in type II atelocollagen showed a 180% increase in new proteoglycan synthesis (p<0.05). The rabbit IVD cells with Ad/BMP-2 and that were cultured in type I atelocollagen showed a 70% increase in new proteoglycan synthesis, while the rabbit IVD cells with Ad/BMP-2 and that were cultured in type II atelocollagen showed a 95% increase (p<0.05). Rabbit IVD cells with Ad/TGF-beta1 and Ad/BMP-2 and that were cultured in type I and II atelocollagen demonstrated increased collagen type I and II mRNA expressions without an osteocalcin mRNA expression (p<0.05). CONCLUSION: Cell and gene therapy in an atelocollagen scaffold provided a efficient mechanism for chondrogenic matrix regeneration of rabbit IVD cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 49~56 | - |
dc.relation.isPartOf | Journal of Korean Society of Spine Surgery | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 아텔로콜라겐 지지체, 유전자 치료, 배양된 수핵세포를 이용한 조직 공학적 추간판의 재생 | - |
dc.title.alternative | Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학) | - |
dc.contributor.googleauthor | Hak-Sun Kim | - |
dc.contributor.googleauthor | Kwang-Il Lee | - |
dc.contributor.googleauthor | Hyang Kim | - |
dc.contributor.googleauthor | Un-Hye Kwon | - |
dc.contributor.googleauthor | Mi-Ran Nam | - |
dc.contributor.googleauthor | Ju-Woong Jang | - |
dc.contributor.googleauthor | In-Je Cho | - |
dc.contributor.googleauthor | Boram Kim | - |
dc.contributor.googleauthor | Hwan-Mo Lee | - |
dc.contributor.googleauthor | Seong-Hwan Moon | - |
dc.identifier.doi | 10.4184/jkss.2010.17.2.49 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00229 | - |
dc.contributor.localId | A00509 | - |
dc.contributor.localId | A01093 | - |
dc.contributor.localId | A01106 | - |
dc.contributor.localId | A01249 | - |
dc.contributor.localId | A01365 | - |
dc.contributor.localId | A02672 | - |
dc.contributor.localId | A03333 | - |
dc.relation.journalcode | J01549 | - |
dc.subject.keyword | Intervertebral disc | - |
dc.subject.keyword | Collagen | - |
dc.subject.keyword | Scaffold | - |
dc.subject.keyword | TGF-β1 | - |
dc.subject.keyword | BMP-2 | - |
dc.contributor.alternativeName | Kwon, Un Hye | - |
dc.contributor.alternativeName | Kim, Bo Ram | - |
dc.contributor.alternativeName | Kim, Hak Sun | - |
dc.contributor.alternativeName | Kim, Hyang | - |
dc.contributor.alternativeName | Nam, Mi Ran | - |
dc.contributor.alternativeName | Moon, Seong Hwan | - |
dc.contributor.alternativeName | Lee, Kwang Il | - |
dc.contributor.alternativeName | Lee, Hwan Mo | - |
dc.contributor.affiliatedAuthor | Kwon, Un Hye | - |
dc.contributor.affiliatedAuthor | Kim, Bo Ram | - |
dc.contributor.affiliatedAuthor | Kim, Hak Sun | - |
dc.contributor.affiliatedAuthor | Kim, Hyang | - |
dc.contributor.affiliatedAuthor | Nam, Mi Ran | - |
dc.contributor.affiliatedAuthor | Moon, Seong Hwan | - |
dc.contributor.affiliatedAuthor | Lee, Kwang Il | - |
dc.contributor.affiliatedAuthor | Lee, Hwan Mo | - |
dc.citation.volume | 17 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 49 | - |
dc.citation.endPage | 56 | - |
dc.identifier.bibliographicCitation | Journal of Korean Society of Spine Surgery, Vol.17(2) : 49-56, 2010 | - |
dc.identifier.rimsid | 52155 | - |
dc.type.rims | ART | - |
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