101 271

Cited 5 times in

A report of cat scratch disease in Korea confirmed by PCR amplification of the 16S-23S rRNA intergenic region of Bartonella henselae

Authors
 Borum Suh  ;  Jin-Kyoung Chun  ;  Dongeun Yong  ;  Yang Soon Lee  ;  Seok Hoon Jeong  ;  Woo Ick Yang  ;  Dong Soo Kim 
Citation
 KOREAN JOURNAL OF LABORATORY MEDICINE , Vol.30(1) : 34-37, 2010 
Journal Title
 KOREAN JOURNAL OF LABORATORY MEDICINE 
ISSN
 1598-6535 
Issue Date
2010
MeSH
Animals ; Bartonella henselae/genetics ; Bartonella henselae/isolation & purification* ; Cat-Scratch Disease/complications ; Cat-Scratch Disease/diagnosis* ; Cats ; Child ; Dogs ; Female ; Humans ; Lymphadenitis/complications ; Polymerase Chain Reaction* ; RNA, Ribosomal, 16S/genetics* ; RNA, Ribosomal, 23S/genetics* ; Republic of Korea ; Sequence Analysis, DNA
Abstract
We report a case of cat scratch disease in an 8-yr-old girl who presented with fever and enlargement of both axillary lymph nodes. Both aerobic and anaerobic cultures of the lymph node aspirate were negative for microbial growth. Gram staining and Warthin-Starry silver staining did not reveal any organism. Purified DNA from the PCR-amplicon of the 16S-23S rRNA intergenic region was sequenced and showed 99.7% identity with the corresponding sequence of Bartonella henselae strain Houston-1. Our findings suggest that the internal transcribed spacer is a reliable region for PCR identification of Bartonella species. In patients with lymphadenitis, a history of contact with cats or dogs necessitates the use of diagnostic approaches that employ not only the conventional staining and culture but also molecular methods to detect B. henselae
Files in This Item:
T201001797.pdf Download
DOI
10.3343/kjlm.2010.30.1.34
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Soo(김동수)
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Yang Soon(이양순)
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101161
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse