1 496

Cited 88 times in

Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.

 Da-Woon Jung  ;  Zhong Min Che  ;  Jinmi Kim  ;  Kyungshin Kim  ;  Ki-Yeol Kim  ;  Darren Williams  ;  Jin Kim 
 INTERNATIONAL JOURNAL OF CANCER, Vol.127(2) : 332-344, 2010 
Journal Title
Issue Date
Antibodies, Neutralizing/pharmacology ; Biomarkers, Tumor/genetics ; Biomarkers, Tumor/metabolism* ; Blotting, Western ; Carcinoma, Squamous Cell/genetics ; Carcinoma, Squamous Cell/metabolism ; Carcinoma, Squamous Cell/pathology* ; Cell Adhesion ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Chemokine CCL7/immunology ; Chemokine CCL7/metabolism* ; Culture Media, Conditioned/pharmacology ; Enzyme-Linked Immunosorbent Assay ; Fibroblasts/metabolism* ; Fibroblasts/pathology ; Gene Expression Profiling ; Humans ; Immunoenzyme Techniques ; Mouth Neoplasms/genetics ; Mouth Neoplasms/metabolism ; Mouth Neoplasms/pathology* ; Oligonucleotide Array Sequence Analysis ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Stromal Cells/metabolism* ; Stromal Cells/pathology ; Wound Healing
CCL7 ; IL‐1α ; chemokine ; carcinoma‐associated fibroblasts ; oral squamous cell carcinoma ; crosstalk
Recent studies have shown that stromal fibroblasts have a more profound influence on the initiation and progression of carcinoma than was previously appreciated. This study aimed at investigating the reciprocal relationship between cancer cells and their associated fibroblasts at both the molecular and cellular level in oral squamous cell carcinoma (OSCC). To identify key molecular regulators expressed by carcinoma-associated fibroblasts (CAF) that promote cancer cell invasion, microarrays were performed by comparing cocultured OSCC cells and CAF with monoculture controls. Microarray and real-time PCR analysis identified marked upregulation of the chemokine (C-C motif) ligand 7 (CCL7) in cocultured CAF. ELISA showed an elevated level of CCL7 secretion from CAF stimulated by coculture with OSCC cells. CCL7 promoted the invasion and migration of OSCC cells, and the invasiveness was inhibited by treatment with CCL7 neutralizing antibody. OSCC cells were shown to express CCR1, CCR2 and CCR3, receptors for CCL7, by RT-PCR. In addition, treatment with anti-CCR1 or anti-CCR3 antibody inhibited CCL7-induced OSCC cell migration, implicating that CCL7 promotes cancer cell migration through CCR1 and CCR3 on OSCC cells. Cytokine antibody array analysis of the supernatant from OSCC cell culture revealed that interleukin-1alpha was an inducer of CCL7 secretion by CAF. This study confirms the reciprocal relationship of the molecular crosstalk regulating the invasion of OSCC and describes new potential targets for future therapy.
Full Text
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Orofacial Pain and Oral Medicine (구강내과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kim, Ki Yeol(김기열) ORCID logo https://orcid.org/0000-0001-5357-1067
Kim, Jin(김진)
Kim, Jin Mi(김진미)
Jung, Da Woon(정다운)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.