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Elevated HDL2-paraoxonase and reduced CETP activity are associated with a dramatically lower ratio of LDL-cholesterol/total cholesterol in a hypercholesterolemic and hypertriglyceridemic patient.

Authors
 Ji-Hye Lee  ;  Jung-Heun Park  ;  Sang-Hak Lee  ;  Jae-Ryong Kim  ;  Kyung-Hyun Cho 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, Vol.25(6) : 945-951, 2010 
Journal Title
 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 
ISSN
 1107-3756 
Issue Date
2010
MeSH
Antioxidants/metabolism ; Apolipoprotein A-I/metabolism ; Aryldialkylphosphatase/metabolism* ; Blotting, Western ; Cholesterol Ester Transfer Proteins/metabolism* ; Cholesterol, HDL/blood ; Cholesterol, HDL/metabolism ; Cholesterol, HDL/ultrastructure ; Cholesterol, LDL/blood ; Cholesterol, LDL/metabolism* ; Cholesterol, LDL/ultrastructure ; Female ; Humans ; Hypercholesterolemia/blood ; Hypercholesterolemia/complications* ; Hypercholesterolemia/enzymology* ; Hypertriglyceridemia/blood ; Hypertriglyceridemia/complications* ; Hypertriglyceridemia/enzymology* ; Iron/metabolism ; Middle Aged ; Oxidation-Reduction
Keywords
apolipoprotein A-I ; dyslipidemia ; cholesteryl ester transfer protein ; paraoxonase
Abstract
A female patient (64 years of age; body mass index, 26) had a markedly and relatively low low-density lipoprotein-cholesterol (LDL-C) level (97 mg/dl) despite high serum total cholesterol (TC) (331 mg/dl) and triacylglyceride levels (307 mg/dl). Since the expected LDL-C was 222 mg/dl, there was a significant difference between the calculation and measurement based on direct enzyme assay. Only 30% of serum cholesterol was associated with LDL-C in this patient. To determine the basis for the markedly low LDL-C/TC ratio, we isolated and analyzed lipoproteins from the patient as well as age- and gender-matched controls. The patient had lowered serum CETP activity and elevated paraoxonase activity with GOT and GPT values in the normal range. The very low-density lipoprotein particles from the patient were larger than those of the controls and enriched with lipid and protein, while the LDL from the patient (LDL-P) had a lower particle number and protein content than the controls. The LDL-P was more resistant to cupric ion-mediated oxidation. HDL2 from the patient (HDL2-P) had highly enhanced paraoxonase activity and antioxidant ability. The patient had a 1.5-fold higher level of apolipoprotein (apo) A-I expression in HDL2. ApoA-I in HDL2 and HDL3 from the patient showed no fragmentation, while the control had fragmented bands (17 and 21 kDa) in the HDL. The HDL2-P also had a larger particle size and greater protein content with less lipid content. HDL3-associated cholesteryl ester transfer protein was reduced in the patient, although the particle size was similar to the controls. In conclusion, a patient who had a markedly lower LDL-C/TC ratio despite hyperlipidemia associated with higher paraoxonase activity, higher apoA-I level and lower CETP activity without fragmentation of apoA-I in the HDL fraction is presented. The enhanced antioxidant and anti-inflammatory activity of HDL might contribute to the low LDL-C/TC ratio in this patient.
Full Text
http://www.spandidos-publications.com/ijmm/25/6/945?text=abstract
DOI
10.3892/ijmm_00000426
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Sang Hak(이상학) ORCID logo https://orcid.org/0000-0002-4535-3745
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100932
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