Cited 70 times in
Characterization, in vitro cytotoxicity assessment, and in vivo visualization of multimodal, RITC-labeled, silica-coated magnetic nanoparticles for labeling human cord blood-derived mesenchymal stem cells
DC Field | Value | Language |
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dc.contributor.author | 이종은 | - |
dc.date.accessioned | 2015-04-23T16:37:42Z | - |
dc.date.available | 2015-04-23T16:37:42Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1549-9634 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100929 | - |
dc.description.abstract | Live imaging is a powerful technique that can be used to characterize the fate and location of stem cells in animal models. Here we investigated the characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, MNPs@SiO2(RITC). We also conducted various in vivo-uptake tests with nanoparticle-labeled human MSCs. MNPs@SiO2(RITC) showed photostability against ultraviolet light exposure and were nontoxic to human MSCs, based on the MTT, apoptosis, and cell cycle arrest assays. In addition, MNPs@SiO2(RITC) did not affect the surface phenotype or morphology of human MSCs. We also demonstrated that MNPs@SiO2(RITC) have stable retention properties in MSCs in vitro. Furthermore, using optical and magnetic resonance imaging, we successfully detected a visible signal from labeled human MSCs that were transplanted into NOD.CB17-Prkdc(SCID) (NOD-SCID) mice. These results demonstrate that MNPs@SiO2(RITC) are biocompatible and useful tools for human MSC labeling and bioimaging. FROM THE CLINICAL EDITOR: The characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, RITC were investigated in this study. RITC showed photostability against ultraviolet light exposure and was nontoxic to human MSCs. Using both optical and magnetic resonance imaging, successful detection of signal from labeled human MSCs transplanted into mice is demonstrated. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 263~276 | - |
dc.relation.isPartOf | NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Apoptosis/drug effects | - |
dc.subject.MESH | Cell Survival/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Contrast Media/chemistry | - |
dc.subject.MESH | Drug Carriers/adverse effects | - |
dc.subject.MESH | Drug Carriers/chemistry* | - |
dc.subject.MESH | Fetal Blood/cytology* | - |
dc.subject.MESH | Fetal Blood/drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Image Enhancement/methods | - |
dc.subject.MESH | MagneticResonance Imaging/methods* | - |
dc.subject.MESH | Magnetics | - |
dc.subject.MESH | MesenchymalStromalCells/cytology* | - |
dc.subject.MESH | MesenchymalStromalCells/drug effects | - |
dc.subject.MESH | Nanoparticles/adverse effects* | - |
dc.subject.MESH | Rhodamines*/adverse effects | - |
dc.subject.MESH | Silicon Dioxide/chemistry* | - |
dc.subject.MESH | Staining andLabeling/methods | - |
dc.title | Characterization, in vitro cytotoxicity assessment, and in vivo visualization of multimodal, RITC-labeled, silica-coated magnetic nanoparticles for labeling human cord blood-derived mesenchymal stem cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anatomy (해부학) | - |
dc.contributor.googleauthor | Ki-Soo Park | - |
dc.contributor.googleauthor | Jinsung Tae | - |
dc.contributor.googleauthor | Bongkum Choi | - |
dc.contributor.googleauthor | Young-Seok Kim | - |
dc.contributor.googleauthor | Cheol Moon | - |
dc.contributor.googleauthor | Sa-Hyun Kim | - |
dc.contributor.googleauthor | Han-Sin Lee | - |
dc.contributor.googleauthor | Jinhyun Kim | - |
dc.contributor.googleauthor | Junsung Kim | - |
dc.contributor.googleauthor | Jaeberm Park | - |
dc.contributor.googleauthor | Jung-Hee Lee | - |
dc.contributor.googleauthor | Jong Eun Lee | - |
dc.contributor.googleauthor | Jae-Won Joh | - |
dc.contributor.googleauthor | Sungjoo Kim | - |
dc.identifier.doi | 10.1016/j.nano.2009.07.005 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03146 | - |
dc.relation.journalcode | J02934 | - |
dc.identifier.eissn | 1549-9642 | - |
dc.identifier.pmid | 19699324 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S154996340900166X | - |
dc.subject.keyword | Biocompatibility | - |
dc.subject.keyword | Mesenchymal stem cells | - |
dc.subject.keyword | Magnetic resonance imaging | - |
dc.subject.keyword | Nanoparticles | - |
dc.subject.keyword | Stem cells | - |
dc.contributor.alternativeName | Lee, Jong Eun | - |
dc.contributor.affiliatedAuthor | Lee, Jong Eun | - |
dc.citation.volume | 6 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 263 | - |
dc.citation.endPage | 276 | - |
dc.identifier.bibliographicCitation | NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, Vol.6(2) : 263-276, 2010 | - |
dc.identifier.rimsid | 54061 | - |
dc.type.rims | ART | - |
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