Cited 9 times in
Proliferation of pancreatic endocrine cells using disaggregation-expansion-reaggregation technology in isolated rat islets
DC Field | Value | Language |
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dc.contributor.author | 정진호 | - |
dc.contributor.author | 조유리 | - |
dc.contributor.author | 주동진 | - |
dc.contributor.author | 주만기 | - |
dc.contributor.author | 허규하 | - |
dc.contributor.author | 김명수 | - |
dc.contributor.author | 김유선 | - |
dc.contributor.author | 김준예 | - |
dc.date.accessioned | 2015-04-23T16:37:20Z | - |
dc.date.available | 2015-04-23T16:37:20Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0041-1345 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100918 | - |
dc.description.abstract | Donor scarcity is a major obstacle for clinical islet transplantation. Hence, the effective use of the limited number of available islets is necessary for successful islet transplantation. We have developed a new technology that could produce pseudo-islets. Morphologic and functional evaluation was performed to test the feasibility of using these cells for transplantation. A 3-step procedure known as disaggregation-expansion-reaggregation (DER) was employed for pseudo-islet preparation. Islets isolated from 200 to 250-g male Lewis rats by collagenase digestion were separated into single cells by trypsinization. These pancreatic endocrine cells (PECs) were expanded by serial passages in culture before being aggregated at a high cell-density in a suspended state. After DER, cells were morphologically analyzed over time, and gene expression evaluated by reverse transcriptase polymerase chain reaction (RT-PCR). Through expansion by passage for 2 weeks in continuous cultures, approximately 1 million PECs were recovered after aggregation. By phase-contrast microscopy, they presented with spherical shapes and similar sizes compared with naïve islets (50-800 microm). RT-PCR results indicated expression of insulin, glucagon, and pancreatic and duodenal homeobox gene 1, which were observed in primary isolated islets as well. The insulin secretion capacity of pseudo-islets was confirmed by enzyme-linked immunosorbent assay. In conclusion, PECs treated with DER showed potential to serve as a cell source for pseudo-islet generation after in vitro cellular expansion. These cells were both morphologically and genetically similar to naïve islets. Our new technique could be a potential method to overcome the scarcity of donor islets in the near future. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 907~910 | - |
dc.relation.isPartOf | TRANSPLANTATION PROCEEDINGS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Aggregation/physiology* | - |
dc.subject.MESH | Centrifugation, Density Gradient/methods | - |
dc.subject.MESH | DNA Primers | - |
dc.subject.MESH | Glucagon/genetics | - |
dc.subject.MESH | Glucose/pharmacology | - |
dc.subject.MESH | Homeodomain Proteins/genetics | - |
dc.subject.MESH | Insulin/genetics | - |
dc.subject.MESH | Isletsof Langerhans/cytology* | - |
dc.subject.MESH | Isletsof Langerhans/drug effects | - |
dc.subject.MESH | Isletsof Langerhans/secretion | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Microscopy, Phase-Contrast/methods | - |
dc.subject.MESH | RNA/genetics | - |
dc.subject.MESH | RNA/isolation & purification | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Inbred Lew | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Trans-Activators/genetics | - |
dc.title | Proliferation of pancreatic endocrine cells using disaggregation-expansion-reaggregation technology in isolated rat islets | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | J.H. Jeong | - |
dc.contributor.googleauthor | J.-I. Lee | - |
dc.contributor.googleauthor | M.K. Ju | - |
dc.contributor.googleauthor | D.J. Joo | - |
dc.contributor.googleauthor | K.H. Huh | - |
dc.contributor.googleauthor | M.S. Kim | - |
dc.contributor.googleauthor | J.Y. Kim | - |
dc.contributor.googleauthor | Y. Cho | - |
dc.contributor.googleauthor | Y.S. Kim | - |
dc.identifier.doi | 10.1016/j.transproceed.2010.02.044 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00424 | - |
dc.contributor.localId | A03749 | - |
dc.contributor.localId | A03872 | - |
dc.contributor.localId | A03948 | - |
dc.contributor.localId | A03949 | - |
dc.contributor.localId | A04344 | - |
dc.contributor.localId | A00785 | - |
dc.contributor.localId | A00956 | - |
dc.relation.journalcode | J02755 | - |
dc.identifier.eissn | 1873-2623 | - |
dc.identifier.pmid | 20430201 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0041134510002599 | - |
dc.contributor.alternativeName | Jeong, Jin Ho | - |
dc.contributor.alternativeName | Cho, Yu Ri | - |
dc.contributor.alternativeName | Joo, Dong Jin | - |
dc.contributor.alternativeName | Joo, Man Ki | - |
dc.contributor.alternativeName | Huh, Kyu Ha | - |
dc.contributor.alternativeName | Kim, Myoung Soo | - |
dc.contributor.alternativeName | Kim, Yu Seun | - |
dc.contributor.alternativeName | Kim, Joon Ye | - |
dc.contributor.affiliatedAuthor | Kim, Myoung Soo | - |
dc.contributor.affiliatedAuthor | Jeong, Jin Ho | - |
dc.contributor.affiliatedAuthor | Cho, Yu Ri | - |
dc.contributor.affiliatedAuthor | Joo, Dong Jin | - |
dc.contributor.affiliatedAuthor | Joo, Man Ki | - |
dc.contributor.affiliatedAuthor | Huh, Kyu Ha | - |
dc.contributor.affiliatedAuthor | Kim, Yu Seun | - |
dc.contributor.affiliatedAuthor | Kim, Joon Ye | - |
dc.citation.volume | 42 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 907 | - |
dc.citation.endPage | 910 | - |
dc.identifier.bibliographicCitation | TRANSPLANTATION PROCEEDINGS, Vol.42(3) : 907-910, 2010 | - |
dc.identifier.rimsid | 54053 | - |
dc.type.rims | ART | - |
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