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Can blood components with age-related changes influence the ageing of endothelial cells?

 Ji Yeon Noh  ;  Sang Ho Oh  ;  Ju Hee Lee  ;  Yeon Sook Kwon  ;  Dong Jin Ryu  ;  Kwang Hoon Lee 
 EXPERIMENTAL DERMATOLOGY, Vol.19(4) : 339-346, 2010 
Journal Title
Issue Date
Actins/metabolism ; Adult ; Aged ; Aging/blood* ; Aging/physiology* ; Annexin A5/metabolism ; Blotting, Western ; Cellular Senescence/physiology* ; Cofilin 1/metabolism ; Down-Regulation/physiology ; Drosophila Proteins/metabolism ; Electrophoresis, Gel, Two-Dimensional ; Endothelial Cells/cytology ; Endothelial Cells/metabolism* ; Humans ; Leukocytes, Mononuclear/metabolism* ; Microfilament Proteins/metabolism ; Middle Aged ; Neoplasm Proteins/metabolism ; Nuclear Proteins/metabolism ; Protein Disulfide-Isomerases/metabolism ; Proteins/analysis ; Proteins/metabolism ; Proteomics/methods ; Proto-Oncogene Proteins p21(ras)/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Serum/physiology* ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Transcription Factors/metabolism ; Up-Regulation/physiology ; Vimentin/metabolism ; Young Adult
ageing ; cytoskeletal regulator ; human dermalmicrovascular endothelial cells ; tumor suppressor
Research on vascular endothelial cell ageing helps elucidate the pathogenesis of diseases associated with cell ageing. To investigate endothelial senescence, we used 2-DE coupled with MS to perform a proteomic analysis of: (i) peripheral blood mononuclear cells (PBMCs) from donors in their 20 s ('young') or 60 s ('old') and (ii) human dermal microvascular endothelial cells (HDMECs) treated with sera from young and old donors. Identified proteins could be classified into several functional categories: (i) cytoskeletal regulators: CapG and cofilin 1; (ii) stress response and signal pathway proteins: TXNDC5 and RSU-1; and (iii) apoptosis proteins: Annexin V. We confirmed by Western blot a decrease of RSU-1, CapG and TXNDC5 in PBMCs from old donors. RSU-1, which regulates signal transduction of the downstream Ras, showed decreased mRNA and protein levels in PBMCs from old donors and decreased mRNA levels in HDMECs treated with sera from old donors. In addition, Ras protein levels were increased in PBMCs from old donors. These data indicate that reduced RSU-1 might induce Ras expression, which subsequently could provoke Ras-induced senescence. In conclusion, our data suggest that blood components that exhibit age-related changes, such as alterations in cytoskeletal regulators and stress proteins, may be associated with endothelial cell ageing.
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1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Oh, Sang Ho(오상호) ORCID logo https://orcid.org/0000-0002-4477-1400
Lee, Kwang Hoon(이광훈)
Lee, Ju Hee(이주희) ORCID logo https://orcid.org/0000-0002-1739-5956
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