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Renal capsule as a stem cell niche.

Authors
 Hyeong-Cheon Park  ;  Kaoru Yasuda  ;  Mei-Chuan Kuo  ;  Jie Ni  ;  Brian Ratliff  ;  Praveen Chander  ;  Michael S. Goligorsky 
Citation
 AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, Vol.298(5) : 1254-1262, 2010 
Journal Title
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
ISSN
 1931-857X 
Issue Date
2010
MeSH
Animals ; Bowman Capsule/cytology* ; Bowman Capsule/physiology* ; Cell Differentiation/physiology ; Cell Movement/physiology ; Cells, Cultured ; Immunophenotyping ; In Vitro Techniques ; Kidney/cytology ; Kidney/physiology ; Mesenchymal Stromal Cells/cytology* ; Mesenchymal Stromal Cells/physiology* ; Mice ; Mice, Inbred Strains ; Models, Animal ; Regeneration/physiology ; Stem Cell Niche/physiology*
Keywords
renal mesenchymal stem cells ; cell migration ; renal ischemia
Abstract
Renal resident stem cells were previously reported within the renal tubules and papillary area. The aim of the present study was to determine whether renal capsules harbor stem cells and whether this pool can be recruited to the renal parenchyma after ischemic injury. We demonstrated the presence of label-retaining cells throughout the renal capsule, at a density of ∼10 cells/mm(2), and their close apposition to the blood vessels. By flow cytometry, in vitro cultured cells derived from the renal capsule were positive for mesenchymal stem cell (MSC) markers (CD29+, vimentin+, Sca-1+, nestin+) but did not express hematopoietic and endothelial stem cell markers. Moreover, renal capsule-derived cells also exhibited self-renewal, clonogenicity, and multipotency in differentiation conditions, all favoring stem cell characteristics and identifying them with MSC. In situ labeling of renal capsules with CM-DiI CellTracker demonstrated in vivo a directed migration of CM-DiI-labeled cells to the ischemic renal parenchyma, with the rate of migration averaging 30 μm/h. Decapsulation of the kidneys during ischemia resulted in a modest, but statistically significant, deceleration of recovery of plasma creatinine compared with ischemic kidneys with intact renal capsule. Comparison of these conditions allows the conclusion that renal capsular cells may contribute ∼25-30% of the recovery from ischemia. In conclusion, the data suggest that the renal capsule may function as a novel stem cell niche harboring MSC capable of participating in the repair of renal injury.
Files in This Item:
T201000910.pdf Download
DOI
10.1152/ajprenal.00406.2009
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Hyeong Cheon(박형천) ORCID logo https://orcid.org/0000-0002-1550-0812
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100866
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