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Various penA mutations together with mtrR, porB and ponA mutations in Neisseria gonorrhoeae isolates with reduced susceptibility to cefixime or ceftriaxone

Authors
 Sang-Guk Lee  ;  Hyukmin Lee  ;  Seok Hoon Jeong  ;  Dongeun Yong  ;  Gyung Tae Chung  ;  Yeong Seon Lee  ;  Yunsop Chong  ;  Kyungwon Lee 
Citation
 JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, Vol.65(4) : 669-675, 2010 
Journal Title
 JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 
ISSN
 0305-7453 
Issue Date
2010
MeSH
Anti-Bacterial Agents/pharmacology* ; Antigens, Bacterial/genetics ; Bacterial Proteins/genetics* ; Bacterial Typing Techniques ; Cefixime/pharmacology* ; Ceftriaxone/pharmacology* ; DNA Fingerprinting ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Genotype ; Humans ; Korea ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Mutation, Missense* ; Neisseria gonorrhoeae/drug effects* ; Sequence Analysis, DNA
Keywords
penicillin binding protein 2 ; genotyping ; antibiotic resistance ; cephalosporins
Abstract
OBJECTIVES: To examine mutations within the penA, mtrR, porB, ponA and pilQ genes of Neisseria gonorrhoeae to determine their contribution to cephalosporin resistance. METHODS: A total of 46 N. gonorrhoeae isolates with reduced susceptibility to cefixime or ceftriaxone (MICs > or = 0.12 mg/L) and two susceptible isolates were selected. The full sequence of penA and partial sequences previously reported as hot mutation sites of the other genes were analysed. Genotyping by N. gonorrhoeae multiantigen sequence typing (NG-MAST) was also performed. RESULTS: A mosaic penicillin-binding protein 2 (PBP 2) was found in a single isolate that exhibited the highest cefixime MIC (0.5 mg/L). The majority of the isolates with reduced susceptibility to cephalosporins contained non-mosaic PBP 2 sequences, of which PBP 2 pattern XIII was most common (28/46). All isolates with reduced susceptibility to cephalosporins also had mtrR and porB mutations. Two susceptible isolates had the PBP 2 pattern XIV and an incomplete MtrR protein, which was a new mutation. Isolates with identical PBP 2 patterns comprised multiple NG-MAST sequence types. CONCLUSIONS: Reduced susceptibility of N. gonorrhoeae to ceftriaxone and cefixime was associated with diverse penA mutations, particularly PBP 2 pattern XIII containing an Ala-501-->Val substitution, together with mtrR and porB mutations. The existence of only one strain having the mosaic penA sequence indicated that ceftriaxone and cefixime resistance in Korea is mostly not associated with a mosaic penA sequence. Highly heterogeneous NG-MAST sequence types excluded the clonal expansion of a particular subtype
Files in This Item:
T201000545.pdf Download
DOI
10.1093/jac/dkp505
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Lee, Sang-Guk(이상국) ORCID logo https://orcid.org/0000-0003-3862-3660
Lee, Hyuk Min(이혁민) ORCID logo https://orcid.org/0000-0002-8523-4126
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
Chong, Yun Sop(정윤섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100700
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