Cited 18 times in
BetaPix up-regulates Na+/H+ exchanger 3 through a Shank2-mediated protein-protein interaction
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.contributor.author | 김주영 | - |
dc.contributor.author | 박현우 | - |
dc.contributor.author | 이민구 | - |
dc.contributor.author | 이정수 | - |
dc.date.accessioned | 2015-04-23T16:29:46Z | - |
dc.date.available | 2015-04-23T16:29:46Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100688 | - |
dc.description.abstract | Na(+)/H(+) exchanger 3 (NHE3) plays an important role in neutral Na(+) transport in mammalian epithelial cells. The Rho family of small GTPases and the PDZ (PSD-95/discs large/ZO-1) domain-based adaptor Shank2 are known to regulate the membrane expression and activity of NHE3. In this study we examined the role of betaPix, a guanine nucleotide exchange factor for the Rho GTPase and a strong binding partner to Shank2, in NHE3 regulation using integrated molecular and physiological approaches. Immunoprecipitation and pulldown assays revealed that NHE3, Shank2, and betaPix form a macromolecular complex when expressed heterologously in mammalian cells as well as endogenously in rat colon, kidney, and pancreas. In addition, these proteins co-segregated at the apical surface of rat colonic epithelial cells, as detected by immunofluorescence staining. When expressed in PS120/NHE3 cells, betaPix increased membrane expression and basal activity of NHE3. Interestingly, the effects of betaPix on NHE3 were abolished by cotransfection with dominant-negative Shank2 mutants and by treatment with Clostridium difficile toxin B, a Rho GTPase inhibitor, indicating that Shank2 and Rho GTPases are involved in betaPix-mediated NHE3 regulation. Knockdown of endogenous betaPix by RNA interference decreased Shank2-induced increase of NHE3 membrane expression in HEK 293T cells. These results indicate that betaPix up-regulates NHE3 membrane expression and activity by Shank2-mediated protein-protein interaction and by activating Rho GTPases in the apical regions of epithelial cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 8104~8113 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Polarity/physiology | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Cricetinae | - |
dc.subject.MESH | Endothelial Cells/cytology | - |
dc.subject.MESH | Endothelial Cells/enzymology* | - |
dc.subject.MESH | Fibroblasts/cytology | - |
dc.subject.MESH | Fibroblasts/enzymology | - |
dc.subject.MESH | Guanine Nucleotide Exchange Factors/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Kidney/cytology | - |
dc.subject.MESH | Membrane Proteins/metabolism | - |
dc.subject.MESH | Nerve Tissue Proteins/metabolism* | - |
dc.subject.MESH | Protein Binding/physiology | - |
dc.subject.MESH | Protons | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Rho Guanine Nucleotide Exchange Factors | - |
dc.subject.MESH | Sodium/metabolism | - |
dc.subject.MESH | Sodium-Hydrogen Exchanger 3 | - |
dc.subject.MESH | Sodium-Hydrogen Exchangers/metabolism* | - |
dc.subject.MESH | Up-Regulation/physiology | - |
dc.subject.MESH | rho GTP-Binding Proteins/metabolism | - |
dc.title | BetaPix up-regulates Na+/H+ exchanger 3 through a Shank2-mediated protein-protein interaction | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Won-Joo Kim | - |
dc.contributor.googleauthor | Ji Hyun Lee | - |
dc.contributor.googleauthor | Jihyun Yi | - |
dc.contributor.googleauthor | Yang-Je Cho | - |
dc.contributor.googleauthor | Kyoung Heo | - |
dc.contributor.googleauthor | Sung Hee Lee | - |
dc.contributor.googleauthor | So Won Kim | - |
dc.contributor.googleauthor | Myeong-Kyu Kim | - |
dc.contributor.googleauthor | Kyung Hwan Kim | - |
dc.contributor.googleauthor | Byung In Lee | - |
dc.contributor.googleauthor | Min Goo Lee | - |
dc.identifier.doi | 10.1074/jbc.M109.055079 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00942 | - |
dc.contributor.localId | A00311 | - |
dc.contributor.localId | A01743 | - |
dc.contributor.localId | A02781 | - |
dc.contributor.localId | A03110 | - |
dc.relation.journalcode | J01258 | - |
dc.identifier.eissn | 1083-351X | - |
dc.identifier.pmid | 20080968 | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.alternativeName | Kim, Joo Young | - |
dc.contributor.alternativeName | Park, Hyun Woo | - |
dc.contributor.alternativeName | Lee, Min Goo | - |
dc.contributor.alternativeName | Lee, Jung Soo | - |
dc.contributor.affiliatedAuthor | Kim, Joo Young | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Park, Hyun Woo | - |
dc.contributor.affiliatedAuthor | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Lee, Jung Soo | - |
dc.citation.volume | 285 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 8104 | - |
dc.citation.endPage | 8113 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.285(11) : 8104-8113, 2010 | - |
dc.identifier.rimsid | 37748 | - |
dc.type.rims | ART | - |
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