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Coculture with BJ fibroblast cells inhibits the adipogenesis and lipogenesis in 3T3-L1 cells

Authors
 Hyun Jeong Jeong  ;  Sahng Wook Park  ;  Hojeong Kim  ;  Sang-Kyu Park  ;  Dojun Yoon 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.392(4) : 520-525, 2010 
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN
 0006-291X 
Issue Date
2010
MeSH
3T3-L1 Cells ; Adipocytes/cytology ; Adipocytes/metabolism ; Adipocytes/physiology* ; Adipogenesis* ; Animals ; CCAAT-Enhancer-Binding Proteins/metabolism ; Coculture Techniques ; DNA-Binding Proteins/metabolism ; Fibroblasts ; Lipogenesis* ; Mice ; PPAR gamma/metabolism ; Sterol Regulatory Element Binding Protein 1/metabolism ; Transcription Factors/metabolism
Keywords
Adipogenesis ; Lipogenesis ; Differentiation ; Adipocyte ; Fibroblast ; Coculture
Abstract
Mouse or human fibroblasts are commonly used as feeder cells to prevent differentiation in stem or primary cell culture. In the present study, we addressed whether fibroblasts can affect the differentiation of adipocytes. We found that the differentiation of 3T3-L1 preadipocytes was strongly suppressed when the cells were cocultured with human fibroblast (BJ) cells. BrdU incorporation analysis indicated that mitotic clonal expansion, an early event required for 3T3-L1 cell adipogenesis, was not affected by BJ cells. The 3T3-L1 cell expression levels of peroxisome proliferator-activated receptor gamma2, CCAAT/enhancer-binding protein alpha (C/EBPalpha), sterol regulatory element binding protein-1c, and Krüppel-like factor 15, but not those of C/EBPbeta or C/EBPdelta, were decreased by coculture with BJ cells. When mature 3T3-L1 adipocytes were cocultured with BJ cells, their lipid contents were significantly reduced, with decreased fatty acid synthase expression and increased phosphorylated form of acetyl-CoA carboxylase 1. Our data indicate that coculture with BJ fibroblast cells inhibits the adipogenesis of 3T3-L1 preadipocytes and decreases the lipogenesis of mature 3T3-L1 adipocytes.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X10000951
DOI
10.1016/j.bbrc.2009.12.184
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Sahng Wook(박상욱) ORCID logo https://orcid.org/0000-0002-9594-7074
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100611
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