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Simple method for quantitative analysis of N-linked glycoproteins in hepatocellular carcinoma specimens

Authors
 Hyoung-Joo Lee  ;  Keun Na  ;  Eun-Young Choi  ;  Kyung Sik Kim  ;  Hoguen Kim  ;  Young-Ki Paik 
Citation
 JOURNAL OF PROTEOME RESEARCH, Vol.9(1) : 308-318, 2010 
Journal Title
JOURNAL OF PROTEOME RESEARCH
ISSN
 1535-3893 
Issue Date
2010
MeSH
Amino Acid Sequence ; Antithrombin III/analysis ; Biomarkers, Tumor/blood* ; Carcinoma, Hepatocellular/blood* ; Glycoproteins/blood* ; Humans ; Isotope Labeling ; Liver Neoplasms/blood* ; Molecular Sequence Data ; Peptide Fragments/blood ; Reproducibility of Results ; Tandem Mass Spectrometry/methods ; Vitronectin/blood
Abstract
Changes in N-linked glycan structures are related to the initiation and progression of hepatocellular carcinoma (HCC), one of the most common fatal cancers worldwide. In this study, we describe a simple and an efficient strategy for the selective identification and quantitation of N-linked glycoproteins that does not require extensive enrichment steps prior to MS/MS analysis. With this approach, N-linked glycoprotein differences between the plasma of healthy and HCC patients were selectively quantified after iTRAQ labeling. We identified a total of 14 N-linked glycopeptides with higher expression in HCC patient plasma than in healthy controls (>or=1.5 fold). Additionally, we characterized alterations in the glycan structures of vitronectin (Asn-169, 242) and antithrombin III (Asn-225) that were identified in HCC patient plasma. The intact glycopeptides with native glycan structures were also elevated in HCC tumor tissue. Taken together, these data support the utility of our approach for high throughput global profiling and quantification of the N-linked glycopeptides to identify disease biomarkers.
Full Text
http://pubs.acs.org/doi/abs/10.1021/pr900649b
DOI
10.1021/pr900649b
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Sik(김경식) ORCID logo https://orcid.org/0000-0001-9498-284X
Kim, Hogeun(김호근)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100586
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