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Analysis of Gene Mutations Associated with Antibiotic Resistance in Helicobacter pylori Strains Isolated from Korean Patients.

Authors
 Kim Jung Mogg  ;  Joo Sung Kim  ;  Nayoung Kim  ;  Yeoung-Jeon Kim  ;  In Young Kim  ;  Young Joon Chee  ;  Chul-Hoon Lee  ;  Hyun Chae Jung 
Citation
 Korean Journal of Helicobacter and Upper Gastrointestinal Research, Vol.14(2) : 95-102, 2014 
Journal Title
 Korean Journal of Helicobacter and Upper Gastrointestinal Research 
ISSN
 1738-3331 
Issue Date
2014
MeSH
Clarithromycin/pharmacology* ; Drug Resistance, Bacterial/genetics ; Erythromycin/pharmacology ; Genes, Bacterial ; Genes, rRNA* ; Helicobacter Infections/microbiology ; Helicobacter pylori/drug effects* ; Helicobacter pylori/genetics* ; Humans ; Korea ; Microbial Sensitivity Tests ; Mutation ; Polymerase Chain Reaction/methods ; RNA, Ribosomal, 23S/genetics* ; Ribosomes/drug effects ; Sequence Analysis, DNA/methods
Keywords
clarithromycin ; gene mutations ; Helicobacter pylori ; primary isolates ; resistance ; secondary isolates
Abstract
Although resistance of Helicobacter pylori to clarithromycin is a major cause of failure of eradication therapies, little information is available regarding gene mutations of clarithromycin-resistant primary and secondary H. pylori isolates in Korea. In the present study, we examined gene mutations of H. pylori 238 rRNA responsible for resistance to clarithromycin. DNA sequences of the 238 rRNA gene in 21 primary clarithromycin-resistant and 64 secondary clarithromycin-resistant strains were determined by PCR amplification and nucleotide sequence analyses. Two mutations of the 238 rRNA gene, A2143G and T2182C, were observed in primary clarithromycin-resistant isolates. In secondary isolates, dual mutation of A2143G+T2182C was frequently observed. In addition, A2143G+T2182C+ T2190C, A2143G+T2182C+C2195T, and A2143G+T2182C+A2223G were observed in secondary isolates. Furthermore, macrolide binding was tested on purified ribosomes isolated from T2182C or A2143C mutant strains with $[^{14}C]$erythromycin. Erythromycin binding increased in a dose-dependent manner for the susceptible strain but not for the mutant strains. These results indicate that secondary isolates show a greater variety of 238 rRNA gene mutation types than primary isolates, and triple mutations of secondary isolates are associated with A2143G+T2182C in H. pylori isolated from Korean patients.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Moon, Byung Soo(문병수)
Lee, Yong Chan(이용찬) ORCID logo https://orcid.org/0000-0001-8800-6906
Lim, Hyun Chul(임현철)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100255
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