Cited 21 times in
The Role and Regulatory Mechanism of 14-3-3 Sigma in Human Breast Cancer
DC Field | Value | Language |
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dc.contributor.author | 양우익 | - |
dc.contributor.author | 이종은 | - |
dc.contributor.author | 정우희 | - |
dc.contributor.author | 정준 | - |
dc.date.accessioned | 2015-01-06T17:27:45Z | - |
dc.date.available | 2015-01-06T17:27:45Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1738-6756 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100024 | - |
dc.description.abstract | PURPOSE: 14-3-3 sigma (σ) is considered to be an important tumor suppressor and decreased expression of the same has been reported in many malignant tumors by hypermethylation at its promoter or ubiquitin-mediated proteolysis by estrogen-responsive ring finger protein (Efp). In this study, we investigated the significance of 14-3-3 σ expression in human breast cancer and its regulatory mechanism. METHODS: Efp was silenced using small interfering RNA (siRNA) in the MCF-7 breast cancer cell line in order to examine its influence on the level of 14-3-3 σ protein. The methylation status of the 14-3-3 σ promoter was also evaluated by methylation-specific polymerase chain reaction (PCR). The expression of Efp and 14-3-3 σ in 220 human breast carcinoma tissues was assessed by immunohistochemistry. Other clinicopathological parameters were also evaluated. RESULTS: Silencing Efp in the MCF-7 breast cancer cell line resulted in increased expression of 14-3-3 σ. The Efp-positive human breast cancers were more frequently 14-3-3 σ-negative (60.5% vs. 39.5%). Hypermethylation of 14-3-3 σ was common (64.9%) and had an inverse association with 14-3-3 σ positivity (p=0.072). Positive 14-3-3 σ expression was significantly correlated with poor prognosis: disease-free survival (p=0.008) and disease-specific survival (p=0.009). CONCLUSION: Our data suggests that in human breast cancer, the regulation of 14-3-3 σ may involve two mechanisms: ubiquitin-mediated proteolysis by Efp and downregulation by hypermethylation. However, the inactivation of 14-3-3 σ is probably achieved mainly by hypermethylation. Interestingly, 14-3-3 σ turned out to be a very significant poor prognostic indicator, which is in contrast to its previously known function as a tumor suppressor, suggesting a different role of 14-3-3 σ in breast cancer. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 207~218 | - |
dc.relation.isPartOf | JOURNAL OF BREAST CANCER | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | The Role and Regulatory Mechanism of 14-3-3 Sigma in Human Breast Cancer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | SeungSang Ko | - |
dc.contributor.googleauthor | Ji Young Kim | - |
dc.contributor.googleauthor | Joon Jeong | - |
dc.contributor.googleauthor | Jong Eun Lee | - |
dc.contributor.googleauthor | Woo Ick Yang | - |
dc.contributor.googleauthor | Woo Hee Jung | - |
dc.identifier.doi | 10.4048/jbc.2014.17.3.207 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02300 | - |
dc.contributor.localId | A03671 | - |
dc.contributor.localId | A03727 | - |
dc.contributor.localId | A03146 | - |
dc.relation.journalcode | J01279 | - |
dc.identifier.eissn | 2092-9900 | - |
dc.identifier.pmid | 25320618 | - |
dc.subject.keyword | Breast neoplasms | - |
dc.subject.keyword | Estrogen-responsive finger protein | - |
dc.subject.keyword | Methylation | - |
dc.subject.keyword | SFN protein | - |
dc.contributor.alternativeName | Yang, Woo Ick | - |
dc.contributor.alternativeName | Lee, Jong Eun | - |
dc.contributor.alternativeName | Jung, Woo Hee | - |
dc.contributor.alternativeName | Jeong, Joon | - |
dc.contributor.affiliatedAuthor | Yang, Woo Ick | - |
dc.contributor.affiliatedAuthor | Jung, Woo Hee | - |
dc.contributor.affiliatedAuthor | Jeong, Joon | - |
dc.contributor.affiliatedAuthor | Lee, Jong Eun | - |
dc.citation.volume | 17 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 207 | - |
dc.citation.endPage | 218 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BREAST CANCER, Vol.17(3) : 207-218, 2014 | - |
dc.identifier.rimsid | 55091 | - |
dc.type.rims | ART | - |
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