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A platform technique for growth factor delivery with novel mode of action

DC FieldValueLanguage
dc.contributor.author김남희-
dc.contributor.author김현실-
dc.contributor.author김희진-
dc.contributor.author유주경-
dc.contributor.author육종인-
dc.contributor.author이윤미-
dc.contributor.author차용훈-
dc.contributor.author허종기-
dc.date.accessioned2015-01-06T17:27:19Z-
dc.date.available2015-01-06T17:27:19Z-
dc.date.issued2014-
dc.identifier.issn0142-9612-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/100010-
dc.description.abstractThough growth factors allow tissue regeneration, the trade-off between their effectiveness and adverse effects limits clinical application. The key issues in current growth factor therapy largely derive from initial burst pharmacokinetics, rapid clearance, and proteolytic cleavage resulting in clinical ineffectiveness and diverse complications. While a number of studies have focused on the development of carriers, issues arising from soluble growth factor remain. In this study, we report a prodrug of growth factors constituting a novel mode of action (MoA). To mimic endogenous protein processing in cells, we developed a recombinant BMP-2 polypeptide based on a protein transduction domain (PTD) to transduce the protein into cells followed by furin-mediated protein cleavage and secretion of active growth factor. As proof of concept, a few micrograms scale of PTD-BMP-2 polypeptide sufficed to induce bone regeneration in vivo. As a simple platform, our technique can easily be extended to delivery of BMP-7 and DKK-1 as therapeutics for TGF-β and canonical Wnt signaling, respectively, to suppress the epithelial–mesenchymal transition (EMT), which constitutes a fundamental biological mechanism of many diseases. This technique largely overcomes the limitations of current soluble growth factors and opens the door to next generation growth factor therapeutics.-
dc.description.statementOfResponsibilityopen-
dc.format.extent9888~9896-
dc.relation.isPartOfBIOMATERIALS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBone Morphogenetic Protein 2/genetics*-
dc.subject.MESHBone Morphogenetic Protein 2/metabolism-
dc.subject.MESHBone Morphogenetic Protein 2/pharmacology-
dc.subject.MESHBone Regeneration/drug effects-
dc.subject.MESHCell Line-
dc.subject.MESHDogs-
dc.subject.MESHFemale-
dc.subject.MESHFurin/metabolism-
dc.subject.MESHHumans-
dc.subject.MESHMice-
dc.subject.MESHPeptides/genetics*-
dc.subject.MESHPeptides/metabolism-
dc.subject.MESHPeptides/pharmacology-
dc.subject.MESHRecombinant Fusion Proteins/genetics-
dc.subject.MESHRecombinant Fusion Proteins/metabolism-
dc.subject.MESHRecombinant Fusion Proteins/pharmacology-
dc.subject.MESHRecombinant Proteins/genetics-
dc.subject.MESHRecombinant Proteins/metabolism-
dc.subject.MESHRecombinant Proteins/pharmacology-
dc.subject.MESHRegenerative Medicine-
dc.subject.MESHTransduction, Genetic/methods*-
dc.subject.MESHTransforming Growth Factor beta/genetics*-
dc.subject.MESHTransforming Growth Factor beta/metabolism-
dc.subject.MESHTransforming Growth Factor beta/pharmacology-
dc.subject.MESHWnt Signaling Pathway/drug effects-
dc.titleA platform technique for growth factor delivery with novel mode of action-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentOral Cancer Research Institute (구강종양연구소)-
dc.contributor.googleauthorNam Hee Kim-
dc.contributor.googleauthorYong Hoon Cha-
dc.contributor.googleauthorHyun Sil Kim-
dc.contributor.googleauthorSoo Eon Lee-
dc.contributor.googleauthorJong-Ki Huh-
dc.contributor.googleauthorJung Kook Kim-
dc.contributor.googleauthorJeong Moon Kim-
dc.contributor.googleauthorJoo Kyung Ryu-
dc.contributor.googleauthorHee-Jin Kim-
dc.contributor.googleauthorYoonmi Lee-
dc.contributor.googleauthorSu Yeon Lee-
dc.contributor.googleauthorInsup Noh-
dc.contributor.googleauthorXiao-Yan Li-
dc.contributor.googleauthorStephen J. Weiss-
dc.contributor.googleauthorTae-Ahn Jahng-
dc.contributor.googleauthorJong In Yook-
dc.identifier.doi10.1016/j.biomaterials.2014.08.005-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00360-
dc.contributor.localIdA01121-
dc.contributor.localIdA01225-
dc.contributor.localIdA02512-
dc.contributor.localIdA02536-
dc.contributor.localIdA04000-
dc.contributor.localIdA04365-
dc.contributor.localIdA03019-
dc.relation.journalcodeJ00312-
dc.identifier.eissn1878-5905-
dc.identifier.pmid25218859-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0142961214008928-
dc.subject.keywordGrowth factors-
dc.subject.keywordMode of action (MoA)-
dc.subject.keywordProtein transduction domain (PTD)-
dc.subject.keywordRegenerative medicine-
dc.contributor.alternativeNameKim, Nam Hee-
dc.contributor.alternativeNameKim, Hyun Sil-
dc.contributor.alternativeNameKim, Hee Jin-
dc.contributor.alternativeNameRyu, Ju Kyoung-
dc.contributor.alternativeNameYook, Jong In-
dc.contributor.alternativeNameLee, Yoon Mi-
dc.contributor.alternativeNameCha, Yong Hoon-
dc.contributor.alternativeNameHuh, Jong Ki-
dc.contributor.affiliatedAuthorKim, Nam Hee-
dc.contributor.affiliatedAuthorKim, Hyun Sil-
dc.contributor.affiliatedAuthorKim, Hee Jin-
dc.contributor.affiliatedAuthorRyu, Ju Kyoung-
dc.contributor.affiliatedAuthorYook, Jong In-
dc.contributor.affiliatedAuthorCha, Yong Hoon-
dc.contributor.affiliatedAuthorHuh, Jong Ki-
dc.contributor.affiliatedAuthorLee, Yoon Mi-
dc.rights.accessRightsfree-
dc.citation.volume35-
dc.citation.number37-
dc.citation.startPage9888-
dc.citation.endPage9896-
dc.identifier.bibliographicCitationBIOMATERIALS, Vol.35(37) : 9888-9896, 2014-
dc.identifier.rimsid54406-
dc.type.rimsART-
Appears in Collections:
2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers

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