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Decrease of GABAergic markers and arc protein expression in the frontal cortex by intraventricular 192 IgG-saporin.

Title
Decrease of GABAergic markers and arc protein expression in the frontal cortex by intraventricular 192 IgG-saporin.
Authors
Jeong D.U;Chang W.S.;Chang J.W;Lee D.;Hwang Y.S.
Issue Date
2011
Journal Title
Dementia and Geriatric Cognitive Disorders
ISSN
1420-8008
Citation
Dementia and Geriatric Cognitive Disorders, Vol.32(1) : 70~78, 2011
Abstract
BACKGROUND/AIMS: Previous studies used 192 IgG-saporin to study cholinergic function because of its facility for selective lesioning; however, results varied due to differences in the methods of administration and behavioral tests used. We examined an animal model of dementia using 192 IgG-saporin to confirm its features before applying this model to research of therapeutic drugs or electrical stimulation techniques. METHODS: Features were verified by the Morris water maze test, immunochemistry, and Western blotting. Animals were examined after intraventricular injection of 192 IgG-saporin (0.63 μg/μl; 6, 8, and 10 μl) or phosphate-buffered saline. RESULTS: In the acquisition phase of the Morris water maze test, the latencies of the injection groups were significantly delayed, but recovered within 1 week. In the probe test, 2 of 4 indices (time in the platform zone and the number of crossings) were significantly different in the 8-μl injection group. Immunohistochemistry revealed the extent of cholinergic destruction. Activity-regulated cytoskeleton-associated protein and glutamate decarboxylase expression significantly decreased in the frontal cortex (8- and 10-μl groups), but not in the hippocampus. CONCLUSION: Spatial memory impairment was associated with cholinergic basal forebrain injury as well as frontocortical GABAergic hypofunction and synaptic plasticity deceleration.
URI
http://www.karger.com/Article/FullText/330741

http://ir.ymlib.yonsei.ac.kr/handle/22282913/94827
DOI
10.1159/000330741
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Neurosurgery
1. 연구논문 > 1. College of Medicine > Medical Research Center
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
Yonsei Authors
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