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Surrogate reporters for enrichment of cells with nuclease-induced mutations

Title
Surrogate reporters for enrichment of cells with nuclease-induced mutations
Authors
Hyojin Kim;Eunji Um;Jin-Soo Kim;Hyongbum Kim;Chorong Jung;Sung-Rae Cho
Issue Date
2011
Journal Title
Nature Methods
ISSN
1548-7091
Citation
Nature Methods, Vol.8(11) : 941~943, 2011
Abstract
Zinc-finger nucleases (ZFNs) and TAL-effector nucleases (TALENs) are powerful tools for creating genetic modifications in eukaryotic cells and organisms. But wild-type and mutant cells that contain genetic modifications induced by these programmable nucleases are often phenotypically indistinguishable, hampering isolation of mutant cells. Here we show that transiently transfected episomal reporters encoding fluorescent proteins can be used as surrogate genes for the efficient enrichment of endogenous gene-modified cells by flow cytometry
URI
http://www.nature.com/nmeth/journal/v8/n11/full/nmeth.1733.html

http://ir.ymlib.yonsei.ac.kr/handle/22282913/94379
DOI
10.1038/nmeth.1733
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Rehabilitation Medicine
Yonsei Authors
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