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Surrogate reporters for enrichment of cells with nuclease-induced mutations

Authors
 Hyojin Kim ; Eunji Um ; Jin-Soo Kim ; Hyongbum Kim ; Chorong Jung ; Sung-Rae Cho 
Citation
 Nature Methods, Vol.8(11) : 941~943, 2011 
Journal Title
 Nature Methods 
ISSN
 1548-7091 
Issue Date
2011
Abstract
Zinc-finger nucleases (ZFNs) and TAL-effector nucleases (TALENs) are powerful tools for creating genetic modifications in eukaryotic cells and organisms. But wild-type and mutant cells that contain genetic modifications induced by these programmable nucleases are often phenotypically indistinguishable, hampering isolation of mutant cells. Here we show that transiently transfected episomal reporters encoding fluorescent proteins can be used as surrogate genes for the efficient enrichment of endogenous gene-modified cells by flow cytometry
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94379
DOI
10.1038/nmeth.1733
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Rehabilitation Medicine
Yonsei Authors
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Link
 http://www.nature.com/nmeth/journal/v8/n11/full/nmeth.1733.html
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