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Surrogate reporters for enrichment of cells with nuclease-induced mutations

Authors
 Hyojin Kim  ;  Eunji Um  ;  Sung-Rae Cho  ;  Chorong Jung  ;  Hyongbum Kim  ;  Jin-Soo Kim 
Citation
 NATURE METHODS, Vol.8(11) : 941-943, 2011 
Journal Title
NATURE METHODS
ISSN
 1548-7091 
Issue Date
2011
MeSH
Base Sequence ; Cell Line ; Deoxyribonucleases/metabolism* ; Humans ; Mutation* ; Zinc Fingers
Abstract
Zinc-finger nucleases (ZFNs) and TAL-effector nucleases (TALENs) are powerful tools for creating genetic modifications in eukaryotic cells and organisms. But wild-type and mutant cells that contain genetic modifications induced by these programmable nucleases are often phenotypically indistinguishable, hampering isolation of mutant cells. Here we show that transiently transfected episomal reporters encoding fluorescent proteins can be used as surrogate genes for the efficient enrichment of endogenous gene-modified cells by flow cytometry
Full Text
http://www.nature.com/nmeth/journal/v8/n11/full/nmeth.1733.html
DOI
10.1038/nmeth.1733
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
Yonsei Authors
Cho, Sung-Rae(조성래) ORCID logo https://orcid.org/0000-0003-1429-2684
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94379
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