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Multiple target loci assembly sequencing (mTAS).

Authors
 Hyojun Han ; Jung-ki Yoon ; Duhee Bang ; Hwangbeom Kim ; Byoung Chul Cho 
Citation
 Analytical Biochemistry, Vol.415(2) : 218~220, 2011 
Journal Title
 Analytical Biochemistry 
ISSN
 0003-2697 
Issue Date
2011
Abstract
Here we present multiple target loci assembly sequencing (mTAS), a method for examining multiple genomic loci in a single DNA sequencing read. The key to the success of mTAS target sequencing is the uniform amplification of multiple target genomic loci into a single DNA fragment using polymerase cycling assembly (PCA). Using this strategy, we successfully collected multiloci sequence information from a single DNA sequencing run. We applied mTAS to examine 29 different sets of human genomic loci, each containing from 2 to 11 single-nucleotide polymorphisms (SNP) present at different exons. We believe mTAS can be used to reduce the cost of Sanger sequencing-based genetic analysis.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/93375
DOI
10.1016/j.ab.2011.04.012
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S0003269711002375
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