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Combination of CYP inhibitor with MEK/ERK inhibitor enhances the inhibitory effect on ERK in BRAF mutant colon cancer cells.

Title
Combination of CYP inhibitor with MEK/ERK inhibitor enhances the inhibitory effect on ERK in BRAF mutant colon cancer cells.
Authors
SUN MIN LIM;JEE WON HWANG;SANG JOON SHIN;JAE KYUNG ROH;HYUN CHEOL CHUNG;SUN YOUNG RHA;KI-YEOL KIM;CHAN HEE PARK;SOO KYUNG BAE;JOONG BAE AHN
Issue Date
2013
Journal Title
Anticancer Research
ISSN
0250-7005
Citation
Anticancer Research, Vol.33(6) : 2499~2508, 2013
Abstract
BACKGROUND/AIM: To investigate mechanisms of discrepancy in response to a MEK/ERK inhibitor, U0126, in KRAS- and BRAF-mutant colorectal cancer cells. MATERIALS AND METHODS: Multiparametric flow cytometry was performed on two colon cancer cell lines, HCT116 and HT29. Cells were treated with U0126, and phospho-specific antibodies were used to monitor ERK signaling. RESULTS: HCT116 and HT29 cells were treated with increasing amounts of U0126. The western blot analysis revealed that by increasing the amount of U0126 resulted in inhibition of phospho-ERK, in HCT116 and to a lesser degree in HT29 cells. Microarray profiling identified CYP1A1 and 1A2 overexpression in HT29 cells and that inhibition of CYP1A1 with α-naphthoflavone and furanfylline restored sensitivity to U0126 in HT29 cells. CONCLUSION: Combination of a CYP inhibitor with MEK/ERK inhibitor enhances the inhibitory effect on ERK in BRAF-mutant colon cancer cells.
URI
http://ar.iiarjournals.org/content/33/6/2499.long

http://ir.ymlib.yonsei.ac.kr/handle/22282913/87808
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
1. 연구논문 > 5. Research Institutes > Oral Cancer Research Institute
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
Yonsei Authors
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