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Conventional and real-time PCR targeting 16S ribosomal RNA for the detection of Mycobacterium tuberculosis complex

Title
Conventional and real-time PCR targeting 16S ribosomal RNA for the detection of Mycobacterium tuberculosis complex
Authors
Choi, Y.;Hong, S-R.;Lee, H.;Shim, T. S.;Cho, S-N.;Lee, G-S.;Wang, H-Y.;Jeon, B-Y.
Issue Date
2015
Journal Title
International Journal of Tuberculosis and Lung Disease
ISSN
1027-3719
Citation
International Journal of Tuberculosis and Lung Disease, Vol.19(9) : 1102~1108, 2015
Abstract
SETTING: Conventional diagnostic methods for tuberculosis (TB) have limited sensitivity and specificity or are time-consuming. OBJECTIVE: 16S rDNA and 16S rRNA of Mycobacterium tuberculosis complex (MTC) were used as targets to develop sensitive and specific polymerase chain reactions (PCRs) to improve the diagnosis of MTC. DESIGN: We developed conventional and real-time PCRs targeting 16S rDNA and rRNA of MTC. RESULTS: PCRs targeting 16S rRNA had a 10-100 times lower limit of detection for M. tuberculosis than PCRs targeting 16S rDNA. The sensitivities of the 16S rDNA PCR, 16S rRNA reverse transcription PCR (RT-PCR), 16S rDNA real-time PCR and 16S rRNA real-time RT-PCR for sputum specimens were respectively 92%, 94.6%, 96% and 100%. Real-time PCR showed no cross-reactivity, but conventional PCR had cross-reactivity to M. avium, M. gastri and M. nonchromogenicum. CONCLUSION: PCRs targeting the 16S rRNA of MTC were more sensitive than those targeting 16S rDNA; 16S rRNA real-time RT-PCR showed the highest sensitivity and specificity for MTC.
URI
http://www.ingentaconnect.com/content/iuatld/ijtld/2015/00000019/00000009/art00020

http://ir.ymlib.yonsei.ac.kr/handle/22282913/141753
DOI
10.5588/ijtld.14.0472
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Microbiology
Yonsei Authors
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