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Conventional and real-time PCR targeting 16S ribosomal RNA for the detection of Mycobacterium tuberculosis complex

Authors
 Choi, Y.  ;  Hong, S-R.  ;  Jeon, B-Y.  ;  Wang, H-Y.  ;  Lee, G-S.  ;  Cho, S-N.  ;  Shim, T. S.  ;  Lee, H. 
Citation
 International Journal of Tuberculosis and Lung Disease, Vol.19(9) : 1102-1108, 2015 
Journal Title
 International Journal of Tuberculosis and Lung Disease 
ISSN
 1027-3719 
Issue Date
2015
Abstract
SETTING: Conventional diagnostic methods for tuberculosis (TB) have limited sensitivity and specificity or are time-consuming. OBJECTIVE: 16S rDNA and 16S rRNA of Mycobacterium tuberculosis complex (MTC) were used as targets to develop sensitive and specific polymerase chain reactions (PCRs) to improve the diagnosis of MTC. DESIGN: We developed conventional and real-time PCRs targeting 16S rDNA and rRNA of MTC. RESULTS: PCRs targeting 16S rRNA had a 10-100 times lower limit of detection for M. tuberculosis than PCRs targeting 16S rDNA. The sensitivities of the 16S rDNA PCR, 16S rRNA reverse transcription PCR (RT-PCR), 16S rDNA real-time PCR and 16S rRNA real-time RT-PCR for sputum specimens were respectively 92%, 94.6%, 96% and 100%. Real-time PCR showed no cross-reactivity, but conventional PCR had cross-reactivity to M. avium, M. gastri and M. nonchromogenicum. CONCLUSION: PCRs targeting the 16S rRNA of MTC were more sensitive than those targeting 16S rDNA; 16S rRNA real-time RT-PCR showed the highest sensitivity and specificity for MTC.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/141753
DOI
10.5588/ijtld.14.0472
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실)
Yonsei Authors
조상래(Cho, Sang Nae)
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Full Text
http://www.ingentaconnect.com/content/iuatld/ijtld/2015/00000019/00000009/art00020
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