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Comparative Analysis of Gene Expression in Normal and Degenerative Human Tendon Cells: Effects of Cyclic Strain

DC FieldValueLanguage
dc.contributor.author이진우-
dc.contributor.author조진선-
dc.contributor.author최우진-
dc.contributor.author박광환-
dc.date.accessioned2015-01-06T17:23:05Z-
dc.date.available2015-01-06T17:23:05Z-
dc.date.issued2014-
dc.identifier.issn1071-1007-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99879-
dc.description.abstractBACKGROUND: Tendinopathy is a clinical problem for which treatment shows mixed results and treatment options are limited. Gene expression signatures early in the mechanotransduction pathway can accurately predict risk and correlate with different clinical outcomes. Studies aimed at elucidating the molecular mechanisms of tendinopathy have focused on small cohorts of genes that show an incomplete picture of the degeneration process. This study compared the effect of cyclic strain on gene expression in tendon cells from normal tendon and chronically painful areas of tendinopathy in 3 patients. METHODS: We measured a panel of mechanotransduction genes and cytoskeletal tensional balance with and without cyclic strain, which disrupts connective tissue synthetic-degradative equilibrium. Normal and degenerative tendons were obtained from patients undergoing surgery to treat chronic painful tendinopathy. A cyclic strain model was established to measure cytoskeletal tensional homeostasis. RESULTS: Prior to cyclic strain, the normal tendon cells exhibited varying patterns of elevated expression of 7 genes compared with degenerative tendon cells. In response to cyclic strain, gene expression of COL1A1, ITGA6, CTNNA1, and CLEC3B was up-regulated in normal tendon cells. Cyclic strain had no effect on degenerative tendon cells. Cyclic strain exacerbated the inhibition of protein synthesis in both cell types, especially in the degenerative tendon cells. CONCLUSION: Alterations in the pattern of gene expression are suggestive of a dynamic equilibrium between synthesis and degradation, whereby cell adhesion molecules are predominantly up-regulated to facilitate cellular reorientation in response to their altered functional environment. CLINICAL RELEVANCE: These data might have future applications, including the identification of markers for early diagnosis, targets for drug design, and indicators for treatment responsiveness and prognosis.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfFOOT & ANKLE INTERNATIONAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCollagen Type I/genetics-
dc.subject.MESHCollagen Type I/metabolism-
dc.subject.MESHFemale-
dc.subject.MESHGene Expression-
dc.subject.MESHHumans-
dc.subject.MESHIntegrin alpha6/genetics-
dc.subject.MESHIntegrin alpha6/metabolism-
dc.subject.MESHLectins, C-Type/genetics-
dc.subject.MESHLectins, C-Type/metabolism-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHStress, Mechanical*-
dc.subject.MESHTendinopathy/genetics*-
dc.subject.MESHTendinopathy/pathology*-
dc.subject.MESHTendons/cytology*-
dc.subject.MESHUp-Regulation-
dc.subject.MESHalpha Catenin/genetics-
dc.subject.MESHalpha Catenin/metabolism-
dc.titleComparative Analysis of Gene Expression in Normal and Degenerative Human Tendon Cells: Effects of Cyclic Strain-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Orthopedic Surgery (정형외과학)-
dc.contributor.googleauthorWoo Jin Choi-
dc.contributor.googleauthorMin Sung Park-
dc.contributor.googleauthorKwang Hwan Park-
dc.contributor.googleauthorJean-Paul Courneya-
dc.contributor.googleauthorJin Sun Cho-
dc.contributor.googleauthorLew C. Schon-
dc.contributor.googleauthorJin Woo Lee-
dc.identifier.doi10.1177/1071100714540885-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03230-
dc.contributor.localIdA03914-
dc.contributor.localIdA04121-
dc.relation.journalcodeJ00902-
dc.identifier.eissn1944-7876-
dc.identifier.pmid24958764-
dc.identifier.urlhttp://fai.sagepub.com/content/35/10/1045.full.pdf+html-
dc.subject.keywordcytoskeletal tensional balance-
dc.subject.keywordmechanical strain-
dc.subject.keywordsuperarray-
dc.subject.keywordtendinopathy-
dc.contributor.alternativeNameLee, Jin Woo-
dc.contributor.alternativeNameCho, Jin Sun-
dc.contributor.alternativeNameChoi, Woo Jin-
dc.contributor.affiliatedAuthorLee, Jin Woo-
dc.contributor.affiliatedAuthorCho, Jin Sun-
dc.contributor.affiliatedAuthorChoi, Woo Jin-
dc.rights.accessRightsfree-
dc.citation.volume35-
dc.citation.number10-
dc.citation.startPage1045-
dc.citation.endPage1056-
dc.identifier.bibliographicCitationFOOT & ANKLE INTERNATIONAL, Vol.35(10) : 1045-1056, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers

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