Cited 19 times in
Computational detection and suppression of sequence-specific off-target phenotypes from whole genome RNAi screens
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김현석 | - |
dc.date.accessioned | 2015-01-06T17:17:40Z | - |
dc.date.available | 2015-01-06T17:17:40Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/99706 | - |
dc.description.abstract | A challenge for large-scale siRNA loss-of-function studies is the biological pleiotropy resulting from multiple modes of action of siRNA reagents. A major confounding feature of these reagents is the microRNA-like translational quelling resulting from short regions of oligonucleotide complementarity to many different messenger RNAs. We developed a computational approach, deconvolution analysis of RNAi screening data, for automated quantitation of off-target effects in RNAi screening data sets. Substantial reduction of off-target rates was experimentally validated in five distinct biological screens across different genome-wide siRNA libraries. A public-access graphical-user-interface has been constructed to facilitate application of this algorithm. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 8214~8222 | - |
dc.relation.isPartOf | NUCLEIC ACIDS RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Algorithms | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Genomics/methods* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | MicroRNAs/chemistry | - |
dc.subject.MESH | RNA Interference* | - |
dc.subject.MESH | RNA, Small Interfering/chemistry | - |
dc.title | Computational detection and suppression of sequence-specific off-target phenotypes from whole genome RNAi screens | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Life Science (의생명과학부) | - |
dc.contributor.googleauthor | Rui Zhong | - |
dc.contributor.googleauthor | Jimi Kim | - |
dc.contributor.googleauthor | Hyun Seok Kim | - |
dc.contributor.googleauthor | Minsoo Kim | - |
dc.contributor.googleauthor | Lawrence Lum | - |
dc.contributor.googleauthor | Beth Levine | - |
dc.contributor.googleauthor | Guanghua Xiao | - |
dc.contributor.googleauthor | Michael A. White | - |
dc.contributor.googleauthor | Yang Xie | - |
dc.identifier.doi | 10.1093/nar/gku306 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01111 | - |
dc.relation.journalcode | J02387 | - |
dc.identifier.eissn | 1362-4962 | - |
dc.identifier.pmid | 24972830 | - |
dc.contributor.alternativeName | Kim, Hyun Seok | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Seok | - |
dc.citation.volume | 42 | - |
dc.citation.number | 13 | - |
dc.citation.startPage | 8214 | - |
dc.citation.endPage | 8222 | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, Vol.42(13) : 8214-8222, 2014 | - |
dc.identifier.rimsid | 57070 | - |
dc.type.rims | ART | - |
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