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Snail1 induced in breast cancer cells in 3D collagen I gel environment suppresses cortactin and impairs effective invadopodia formation

DC Field Value Language
dc.contributor.author강숙희-
dc.contributor.author김백길-
dc.contributor.author조남훈-
dc.date.accessioned2015-01-06T17:11:54Z-
dc.date.available2015-01-06T17:11:54Z-
dc.date.issued2014-
dc.identifier.issn0167-4889-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99518-
dc.description.abstractAlthough an in vitro 3D environment cannot completely mimic the in vivo tumor site, embedding tumor cells in a 3D extracellular matrix (ECM) allows for the study of cancer cell behaviors and the screening of anti-metastatic reagents with a more in vivo-like context. Here we explored the behaviors of MDA-MB-231 breast cancer cells embedded in 3D collagen I. Diverse tumor environmental conditions (including cell density, extracellular acidity, or hypoxia as mimics for a continuous tumor growth) reduced JNKs, enhanced TGFβ1/Smad signaling activity, induced Snail1, and reduced cortactin expression. The reduced JNKs activity blocked efficient formation of invadopodia labeled with actin, cortactin, or MT1-MMP. JNKs inactivation activated Smad2 and Smad4, which were required for Snail1 expression. Snail1 then repressed cortactin expression, causing reduced invadopodia formation and prominent localization of MT1-MMP at perinuclear regions. MDA-MB-231 cells thus exhibited less efficient collagen I degradation and invasion in 3D collagen I upon JNKs inhibition. These observations support a signaling network among JNKs, Smads, Snail1, and cortactin to regulate the invasion of MDA-MB-231 cells embedded in 3D collagen I, which may be targeted during screening of anti-invasion reagents.-
dc.description.statementOfResponsibilityopen-
dc.format.extent2037~2054-
dc.relation.isPartOfBIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHActins/metabolism-
dc.subject.MESHAnimals-
dc.subject.MESHBreast Neoplasms/enzymology-
dc.subject.MESHBreast Neoplasms/metabolism*-
dc.subject.MESHBreast Neoplasms/pathology*-
dc.subject.MESHCattle-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Membrane/metabolism-
dc.subject.MESHCell Movement-
dc.subject.MESHCell Nucleus/metabolism-
dc.subject.MESHCollagen Type I/pharmacology*-
dc.subject.MESHCortactin/genetics-
dc.subject.MESHCortactin/metabolism*-
dc.subject.MESHFemale-
dc.subject.MESHGels-
dc.subject.MESHHumans-
dc.subject.MESHJNK Mitogen-Activated Protein Kinases/antagonists & inhibitors-
dc.subject.MESHJNK Mitogen-Activated Protein Kinases/metabolism-
dc.subject.MESHMatrix Metalloproteinase 14/metabolism-
dc.subject.MESHNeoplasm Invasiveness-
dc.subject.MESHPhosphoserine/metabolism-
dc.subject.MESHProtein Transport-
dc.subject.MESHProto-Oncogene Proteins c-jun/metabolism-
dc.subject.MESHPseudopodia/drug effects-
dc.subject.MESHPseudopodia/metabolism*-
dc.subject.MESHSignal Transduction-
dc.subject.MESHSmad Proteins/metabolism-
dc.subject.MESHSnail Family Transcription Factors-
dc.subject.MESHTranscription Factors/metabolism*-
dc.subject.MESHTranscription, Genetic-
dc.subject.MESHTransforming Growth Factor beta1/metabolism-
dc.subject.MESHTumor Microenvironment/drug effects*-
dc.titleSnail1 induced in breast cancer cells in 3D collagen I gel environment suppresses cortactin and impairs effective invadopodia formation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorMi-Sook Lee-
dc.contributor.googleauthorSudong Kim-
dc.contributor.googleauthorBaek Gil Kim-
dc.contributor.googleauthorCheolhee Won-
dc.contributor.googleauthorSeo Hee Nam-
dc.contributor.googleauthorSuki Kang-
dc.contributor.googleauthorHye-Jin Kim-
dc.contributor.googleauthorMinkyung Kang-
dc.contributor.googleauthorJihye Ryu-
dc.contributor.googleauthorHaeng Eun Song-
dc.contributor.googleauthorDoohyung Lee-
dc.contributor.googleauthorSang-Kyu Ye-
dc.contributor.googleauthorNoo Li Jeon-
dc.contributor.googleauthorTai Young Kim-
dc.contributor.googleauthorNam Hoon Cho-
dc.contributor.googleauthorJung Weon Lee-
dc.identifier.doi10.1016/j.bbamcr.2014.05.007-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00044-
dc.contributor.localIdA00484-
dc.contributor.localIdA03812-
dc.relation.journalcodeJ00291-
dc.identifier.eissn1878-2434-
dc.identifier.pmid24861866-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0167488914001542-
dc.subject.keyword3D collagen-
dc.subject.keywordCortactin-
dc.subject.keywordInvasion-
dc.subject.keywordJNK-
dc.subject.keywordSnail1-
dc.contributor.alternativeNameKang, Suki-
dc.contributor.alternativeNameKim, Baek Gil-
dc.contributor.alternativeNameCho, Nam Hoon-
dc.contributor.affiliatedAuthorKang, Suki-
dc.contributor.affiliatedAuthorKim, Baek Gil-
dc.contributor.affiliatedAuthorCho, Nam Hoon-
dc.rights.accessRightsfree-
dc.citation.volume1843-
dc.citation.number9-
dc.citation.startPage2037-
dc.citation.endPage2054-
dc.identifier.bibliographicCitationBIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, Vol.1843(9) : 2037-2054, 2014-
dc.identifier.rimsid39472-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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