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Cancer-associated fibroblast promote transmigration through endothelial brain cells in three-dimensional in vitro models

DC FieldValueLanguage
dc.contributor.author강숙희-
dc.contributor.author김세훈-
dc.contributor.author이주현-
dc.contributor.author조남훈-
dc.contributor.author김백길-
dc.date.accessioned2015-01-06T17:11:50Z-
dc.date.available2015-01-06T17:11:50Z-
dc.date.issued2014-
dc.identifier.issn0020-7136-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99516-
dc.description.abstractBrain metastases are associated with high morbidity as well as with poor prognosis and survival in breast cancer patients. Despite its clinical importance, metastasis of breast cancer cells through the blood-brain barrier (BBB) is poorly understood. The objective of our study was to investigate whether cancer-associated fibroblasts (CAFs) play crucial roles in breast cancer brain metastasis. Using a cell adhesion assays, in vitro BBB permeability and transmigration assays and soft agar colony formation assays, we investigated the physical roles of CAFs in breast cancer brain metastasis. We also performed immunofluorescence, flow cytometric analysis, Droplet Digital PCR and Simon™ Simple Western System to confirm changes in expression levels. We established two novel three-dimensional (3D) culture systems using a perpendicular slide chamber and applying 3D embedded culture method to reflect brain metastasis conditions. With a newly developed device, CAFs was proven to promote cell adhesion to human brain microvascular endothelial cells, in vitro BBB permeability and transmigration and colony formation of breast cancer cells. Furthermore, CAFs enhanced the invasive migration of breast cancer cells in two kinds of 3D cultures. These 3D models also reliably recapitulate the initial steps of BBB transmigration, micro-metastasis and colonization. Expression of integrin α5β1 and αvβ3, c-MET and α2,6-siayltransferase was increased in breast cancer cells that migrated through the BBB. In conclusion, based on our in vitro BBB and co-culture models, our data suggest that CAFs may play a role in breast cancer brain metastasis.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfINTERNATIONAL JOURNAL OF CANCER-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBiological Transport-
dc.subject.MESHBlood-Brain Barrier-
dc.subject.MESHBlotting, Western-
dc.subject.MESHBrain/metabolism-
dc.subject.MESHBrain/pathology*-
dc.subject.MESHBrain Neoplasms/genetics-
dc.subject.MESHBrain Neoplasms/metabolism-
dc.subject.MESHBrain Neoplasms/secondary*-
dc.subject.MESHBreast Neoplasms/genetics-
dc.subject.MESHBreast Neoplasms/metabolism-
dc.subject.MESHBreast Neoplasms/pathology*-
dc.subject.MESHCell Adhesion-
dc.subject.MESHCell Movement*-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCoculture Techniques-
dc.subject.MESHEndothelium, Vascular/metabolism-
dc.subject.MESHEndothelium, Vascular/pathology*-
dc.subject.MESHFemale-
dc.subject.MESHFibroblasts/metabolism-
dc.subject.MESHFibroblasts/pathology*-
dc.subject.MESHFlow Cytometry-
dc.subject.MESHFluorescent Antibody Technique-
dc.subject.MESHHumans-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHStromal Cells/metabolism-
dc.subject.MESHStromal Cells/pathology*-
dc.subject.MESHTumor Cells, Cultured-
dc.titleCancer-associated fibroblast promote transmigration through endothelial brain cells in three-dimensional in vitro models-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorYoon Pyo Choi-
dc.contributor.googleauthorJoo Hyun Lee-
dc.contributor.googleauthorMing-Qing Gao-
dc.contributor.googleauthorBaek Gil Kim-
dc.contributor.googleauthorSuki Kang-
dc.contributor.googleauthorSe Hoon Kim-
dc.contributor.googleauthorNam Hoon Cho-
dc.identifier.doi10.1002/ijc.28848-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00044-
dc.contributor.localIdA00610-
dc.contributor.localIdA03812-
dc.relation.journalcodeJ01092-
dc.identifier.eissn1097-0215-
dc.identifier.pmid24643985-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/ijc.28848/abstract-
dc.subject.keyword3D culture-
dc.subject.keywordblood-brain barrier-
dc.subject.keywordbrain metastasis-
dc.subject.keywordbreast cancer-
dc.subject.keywordcancer-associated fibroblast-
dc.contributor.alternativeNameKang, Suki-
dc.contributor.alternativeNameKim, Se Hoon-
dc.contributor.alternativeNameLee, Joo Hyun-
dc.contributor.alternativeNameCho, Nam Hoon-
dc.contributor.affiliatedAuthorKang, Suki-
dc.contributor.affiliatedAuthorKim, Se Hoon-
dc.contributor.affiliatedAuthorCho, Nam Hoon-
dc.rights.accessRightsfree-
dc.citation.volume135-
dc.citation.number9-
dc.citation.startPage2024-
dc.citation.endPage2033-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF CANCER, Vol.135(9) : 2024-2033, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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