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Phosphoglucomutase1 is necessary for sustained cell growth under repetitive glucose depletion

Authors
 Eunju Bae  ;  Hee Eun Kim  ;  Eunjin Koh  ;  Kyung-Sup Kim 
Citation
 FEBS LETTERS, Vol.588(17) : 3074-3080, 2014 
Journal Title
 FEBS LETTERS 
ISSN
 0014-5793 
Issue Date
2014
MeSH
Amino Acid Sequence ; Animals ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Dose-Response Relationship, Drug ; Enzyme Stability ; Glucose/deficiency* ; Glucose/pharmacology ; Glucose-6-Phosphate/metabolism ; Glucosephosphates/metabolism ; Glycogen/metabolism ; Humans ; Phosphoglucomutase/chemistry ; Phosphoglucomutase/metabolism* ; Threonine
Keywords
Glycogen ; Glycogenesis ; Glycogenolysis ; Nutritional stress ; Phosphoglucomutase
Abstract
Phosphoglucomutase (PGM)1 catalyzes the reversible conversion reaction between glucose-1-phosphate (G-1-P) and glucose-6-phosphate (G-6-P). Although both G-1-P and G-6-P are important intermediates for glucose and glycogen metabolism, the biological roles and regulatory mechanisms of PGM1 are largely unknown. In this study we found that T553 is obligatory for PGM1 stability and the last C-terminal residue, T562, is critical for its activity. Interestingly, depletion of PGM1 was associated with declined cellular glycogen content and decreased rates of glycogenolysis and glycogenesis. Furthermore, PGM1 depletion suppressed cell proliferation under long-term repetitive glucose depletion. Our results suggest that PGM1 is required for sustained cell growth during nutritional changes, probably through regulating the balance of G-1-P and G-6-P in order to satisfy the cellular demands during nutritional stress.
Full Text
http://www.sciencedirect.com/science/article/pii/S0014579314004967
DOI
10.1016/j.febslet.2014.06.034
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Koh, Eun Jin(고은진) ORCID logo https://orcid.org/0000-0001-8967-6266
Kim, Kyung Sup(김경섭) ORCID logo https://orcid.org/0000-0001-8483-8537
Kim, Hee Eun(김희은)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/99384
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