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The Potential of Endothelial Colony-Forming Cells to Improve Early Graft Loss After Intraportal Islet Transplantation

Authors
 Jung, Hye Seung  ;  Kim, Min Joo  ;  Hong, Shin Hee  ;  Lee, Ye Jin  ;  Kang, Shiane  ;  Lee, Hakmo  ;  Chung, Sung Soo  ;  Park, Joong Shin  ;  Park, Kyong Soo 
Citation
 CELL TRANSPLANTATION, Vol.23(3) : 273-283, 2014 
Journal Title
 CELL TRANSPLANTATION 
ISSN
 0963-6897 
Issue Date
2014
MeSH
Animals ; Blood Glucose/analysis ; Cells, Cultured ; Coculture Techniques/methods ; Diabetes Mellitus, Experimental/surgery* ; Endothelial Cells/cytology* ; Endothelial Cells/immunology ; Graft Rejection/immunology ; Graft Rejection/prevention & control* ; HMGB1 Protein/analysis ; Humans ; Inflammation/immunology ; Islets of Langerhans/cytology* ; Islets of Langerhans/immunology ; Islets of Langerhans Transplantation/immunology ; Islets of Langerhans Transplantation/methods* ; Mice ; Mice, Nude ; Stem Cells/cytology* ; Stem Cells/immunology ; Swine ; Transplantation, Heterologous ; Tumor Necrosis Factor-alpha/analysis
Keywords
Islet transplantation (ITx) ; Instant blood-mediated inflammatory reaction (IBMIR) ; Endothelial colony-forming cells (ECFCs) ; Endothelial progenitor cell ; Early graft loss ; High-mobility group box 1 (HMGB1)
Abstract
Early graft loss in islet transplantation means that a large amount of donor islets is required. Endothelial cells and endothelial colony-forming cells (ECFCs) have been reported to improve instant blood-mediated inflammatory reaction (IBMIR) in vitro. In this study, we examined if ECFC-coated porcine islets would prevent early graft loss in vivo. Human ECFCs were prepared from cord blood and cocultured with islets to make composite grafts. Diabetic nude mice underwent intraportal transplantation. Blood glucose levels were monitored, and morphological examination of the grafts along with analysis of the components of IBMIR and inflammatory reaction were performed with the liver tissues. The ECFC-coated islets significantly decreased blood glucose levels immediately after transplantation compared to the uncoated islets. Composite ECFC islet grafts were observed in the liver sections, associated with a more insulin(+) area compared to that of the uncoated group within 48 h after transplantation. Deposition of CD41a, C5b-9, and CD11b(+) cells was also decreased in the ECFC-coated group. Expression of porcine HMGB1 and mouse TNF-α was increased in the transplantated groups compared to the sham operation group, with a trend of a decreasing trend across the uncoated group, the ECFC-coated group, and the sham group. We demonstrated that the composite ECFC porcine islets transplanted into the portal vein of nude mice improved early graft loss and IBMIR in vivo.
Full Text
http://www.ingentaconnect.com/content/cog/ct/2014/00000023/00000003/art00002?token=00491740dfd3a457e2a46762c6b79217d666a25442e6e5f24673f7b2f27375f2a72752d70
DOI
10.3727/096368912X661364
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Shin Ae(강신애) ORCID logo https://orcid.org/0000-0002-9719-4774
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/99214
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