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The Potential of Endothelial Colony-Forming Cells to Improve Early Graft Loss After Intraportal Islet Transplantation

 Jung, Hye Seung  ;  Kim, Min Joo  ;  Hong, Shin Hee  ;  Lee, Ye Jin  ;  Kang, Shiane  ;  Lee, Hakmo  ;  Chung, Sung Soo  ;  Park, Joong Shin  ;  Park, Kyong Soo 
 CELL TRANSPLANTATION, Vol.23(3) : 273-283, 2014 
Journal Title
Issue Date
Animals ; Blood Glucose/analysis ; Cells, Cultured ; Coculture Techniques/methods ; Diabetes Mellitus, Experimental/surgery* ; Endothelial Cells/cytology* ; Endothelial Cells/immunology ; Graft Rejection/immunology ; Graft Rejection/prevention & control* ; HMGB1 Protein/analysis ; Humans ; Inflammation/immunology ; Islets of Langerhans/cytology* ; Islets of Langerhans/immunology ; Islets of Langerhans Transplantation/immunology ; Islets of Langerhans Transplantation/methods* ; Mice ; Mice, Nude ; Stem Cells/cytology* ; Stem Cells/immunology ; Swine ; Transplantation, Heterologous ; Tumor Necrosis Factor-alpha/analysis
Islet transplantation (ITx) ; Instant blood-mediated inflammatory reaction (IBMIR) ; Endothelial colony-forming cells (ECFCs) ; Endothelial progenitor cell ; Early graft loss ; High-mobility group box 1 (HMGB1)
Early graft loss in islet transplantation means that a large amount of donor islets is required. Endothelial cells and endothelial colony-forming cells (ECFCs) have been reported to improve instant blood-mediated inflammatory reaction (IBMIR) in vitro. In this study, we examined if ECFC-coated porcine islets would prevent early graft loss in vivo. Human ECFCs were prepared from cord blood and cocultured with islets to make composite grafts. Diabetic nude mice underwent intraportal transplantation. Blood glucose levels were monitored, and morphological examination of the grafts along with analysis of the components of IBMIR and inflammatory reaction were performed with the liver tissues. The ECFC-coated islets significantly decreased blood glucose levels immediately after transplantation compared to the uncoated islets. Composite ECFC islet grafts were observed in the liver sections, associated with a more insulin(+) area compared to that of the uncoated group within 48 h after transplantation. Deposition of CD41a, C5b-9, and CD11b(+) cells was also decreased in the ECFC-coated group. Expression of porcine HMGB1 and mouse TNF-α was increased in the transplantated groups compared to the sham operation group, with a trend of a decreasing trend across the uncoated group, the ECFC-coated group, and the sham group. We demonstrated that the composite ECFC porcine islets transplanted into the portal vein of nude mice improved early graft loss and IBMIR in vivo.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Shin Ae(강신애) ORCID logo https://orcid.org/0000-0002-9719-4774
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