353 544

Cited 3 times in

Resolution of Ambiguous HLA Genotyping in Korean by Multi-Group-Specific Sequence-Based Typing

DC Field Value Language
dc.contributor.author김유선-
dc.contributor.author김현숙-
dc.contributor.author박용정-
dc.date.accessioned2015-01-06T17:00:59Z-
dc.date.available2015-01-06T17:00:59Z-
dc.date.issued2014-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99208-
dc.description.abstractPURPOSE: To evaluate a multi-group-specific sequence-based typing (SBT) method for resolving ambiguous results from human leukocyte antigen (HLA) genotyping. MATERIALS AND METHODS:A total of 50 samples that showed ambiguous genotypes for at least two HLA loci from HLA-A, -B, -C and -DRB1 by the conventional SBT assay were evaluated using a new SBT test, the AVITA plus assay. The most likely HLA genotypes for the respective samples considering allele frequencies in Korean were concordant between the AVITA and conventional SBT assays. RESULTS:An average of 3.3 loci among the HLA-A, -B, -C and -DRB1 loci per sample gave results with two or more possible allele combinations with the conventional SBT, and 48 (96.0%) out of 50 showed reduced numbers of possible genotypes for at least one HLA locus with the AVITA. A total of 41, 43, 42, and 38 cases among the 50 samples showed ambiguous results for HLA-A, -B, -C, and -DRB1 typing by the conventional SBT, respectively. The average numbers of possible allele combinations for the respective four HLA loci were 8.2, 6.7, 5.9, and 3.2, and they were reduced to 1.5, 2.2, 4.4, and 1.8, respectively, by the AVITA. Ambiguity was resolved by the AVITA in 33 (80.5%), 31 (72.1%), 17 (40.5%) and 28 (73.7%) samples among the ambiguous cases from the conventional SBT for HLA-A, -B, -C, and -DRB1 typing, respectively. CONCLUSION:The multi-group-specific SBT method considerably reduced the number of ambiguous results, and thus may be useful for accurate HLA typing in clinical laboratories.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1005~1013-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAsian Continental Ancestry Group/genetics-
dc.subject.MESHBase Sequence-
dc.subject.MESHGene Frequency/genetics-
dc.subject.MESHGenotype-
dc.subject.MESHHLA Antigens/genetics*-
dc.subject.MESHHistocompatibility Testing-
dc.subject.MESHHumans-
dc.subject.MESHPolymerase Chain Reaction-
dc.titleResolution of Ambiguous HLA Genotyping in Korean by Multi-Group-Specific Sequence-Based Typing-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthorYongjung Park-
dc.contributor.googleauthorCha Eun Yoon-
dc.contributor.googleauthorOh-Joong Kwon-
dc.contributor.googleauthorYu-Seun Kim-
dc.contributor.googleauthorHyon-Suk Kim-
dc.identifier.doi10.3349/ymj.2014.55.4.1005-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00785-
dc.contributor.localIdA01582-
dc.contributor.localIdA01117-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid24954331-
dc.subject.keywordHuman leukocyte antigen-
dc.subject.keywordambiguity-
dc.subject.keywordhigh resolution-
dc.subject.keywordmulti-group-specific PCR-
dc.subject.keywordsequence-based typing-
dc.contributor.alternativeNameKim, Yu Seun-
dc.contributor.alternativeNameKim, Hyon Suk-
dc.contributor.alternativeNamePark, Yong Jung-
dc.contributor.affiliatedAuthorKim, Yu Seun-
dc.contributor.affiliatedAuthorPark, Yong Jung-
dc.contributor.affiliatedAuthorKim, Hyon Suk-
dc.citation.volume55-
dc.citation.number4-
dc.citation.startPage1005-
dc.citation.endPage1013-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.55(4) : 1005-1013, 2014-
dc.identifier.rimsid55936-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.