Cited 71 times in
Differential Expression of Enzymes Associated with Serine/Glycine Metabolism in Different Breast Cancer Subtypes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 구자승 | - |
dc.contributor.author | 김상겸 | - |
dc.contributor.author | 정우희 | - |
dc.date.accessioned | 2015-01-06T16:53:27Z | - |
dc.date.available | 2015-01-06T16:53:27Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/98967 | - |
dc.description.abstract | PURPOSE: Glycine and serine are well-known, classic metabolites of glycolysis. Here, we profiled the expression of enzymes associated with serine/glycine metabolism in different molecular subtypes of breast cancer and discuss their potential clinical implications. METHODS: We used western blotting and immunohistochemistry to examine five serine-/glycine-metabolism-associated proteins (PHGDH, PSAT, PSPH, SHMT, and GLDC) in six breast cancer cell lines and 709 breast cancer cases using tissue microarray (TMA). RESULTS: PHGDH and PSPH, associated with serine metabolism, were highly expressed in the TNBC cells. GLDC, associated with glycine metabolism, was highly expressed in HER-2-positive MDA-MB-453 and TNBC-related MDA-MB-435S. TMA showed that the TNBC-type breast cancer tissues highly expressed PHGDH, PSPH, and SHMT1, but not the luminal-A-type tissues (p<0.001). PSPH and SHMT1 expression in the tumor stroma of HER-2-type cancers was the highest, but the luminal-A tissues showed the lowest expression (p<0.001). GLDC was most frequently expressed in cancer cells and stroma of the HER-2-positive cancers and least frequently in TNBC (p<0.001). By Cox multivariate analysis, tumor PSPH positivity (hazard ratio [HR]: 2.068, 95% confidence interval [CI]: 1.049-4.079, p = 0.036), stromal PSPH positivity (HR: 2.152, 95% CI: 1.107-4.184, p = 0.024), and stromal SHMT1 negativity (HR: 2.142, 95% CI: 1.219-3.764, p = 0.008) were associated with short overall survival. CONCLUSIONS: Expression of serine-metabolism-associated proteins was increased in TNBC and decreased in the luminal-A cancers. Expression of glycine-metabolism-associated proteins was high in the tumor and stroma of HER-2-positive cancers. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | e101004 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Breast Neoplasms/classification* | - |
dc.subject.MESH | Breast Neoplasms/enzymology* | - |
dc.subject.MESH | Breast Neoplasms/pathology | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Disease-Free Survival | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Glycine/metabolism* | - |
dc.subject.MESH | Glycolysis | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Multivariate Analysis | - |
dc.subject.MESH | Neoplasm Proteins/metabolism | - |
dc.subject.MESH | Phenotype | - |
dc.subject.MESH | Serine/metabolism* | - |
dc.title | Differential Expression of Enzymes Associated with Serine/Glycine Metabolism in Different Breast Cancer Subtypes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학) | - |
dc.contributor.googleauthor | Sang Kyum Kim | - |
dc.contributor.googleauthor | Woo Hee Jung | - |
dc.contributor.googleauthor | Ja Seung Koo | - |
dc.identifier.doi | 10.1371/journal.pone.0101004 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00198 | - |
dc.contributor.localId | A00520 | - |
dc.contributor.localId | A03671 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 24979213 | - |
dc.contributor.alternativeName | Koo, Ja Seung | - |
dc.contributor.alternativeName | Kim, Sang Kyum | - |
dc.contributor.alternativeName | Jung, Woo Hee | - |
dc.contributor.affiliatedAuthor | Koo, Ja Seung | - |
dc.contributor.affiliatedAuthor | Kim, Sang Kyum | - |
dc.contributor.affiliatedAuthor | Jung, Woo Hee | - |
dc.contributor.affiliatedAuthor | 구자승 | - |
dc.citation.volume | 9 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | e101004 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.9(6) : e101004, 2014 | - |
dc.identifier.rimsid | 54315 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.