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Synergistic Restoring Effects of Isoproterenol and Magnesium on KCNQ1-Inhibited Bradycardia Cell Models Cultured in Microelectrode Array
DC Field | Value | Language |
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dc.contributor.author | 박희남 | - |
dc.date.accessioned | 2015-01-06T16:50:19Z | - |
dc.date.available | 2015-01-06T16:50:19Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0008-6312 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/98867 | - |
dc.description.abstract | Objectives: Bradycardia is caused by loss-of-function mutations in potassium channels that regulate phase 3 repolarization of the cardiac action potential. The purpose of this study is to monitor the effects of potassium channel (KCNQ1) inhibition and to evaluate the effects of isoproterenol (ISO) and MgSO4 in restoring sinus rhythm in atrial cells. Methods: Microelectrode array was used to analyze conduction velocity, voltage amplitude and cycle length of atrial cells (HL-1). A combination of ISO and MgSO4 was used to restore sinus rhythm in these cells. Results: mRNA expression levels of KCNQ1 (42.2 vs. 100%, p < 0.0001), connexin 43 (29.6 vs. 100%, p = 0.0033), atrial natriuretic peptide (31.0 vs. 100%, p = 0.0030), cardiac actin (38.2 vs. 100%, p < 0.0001) and α-myosin heavy chain (31.2 vs. 100%, p = 0.00254) were significantly lower in the KCNQ1 gene-inhibited group compared to the control group. When treated with MgSO4 (1 mM) and ISO (10 μM), conduction velocity (0.0208 ± 0.0036 vs. 0.0086 ± 0.0014 m/s, p = 0.0004) and voltage amplitude (1,210.78 ± 65.81 vs. 124.1 ± 13.30 μV, p < 0.0001) were higher, and cycle length (431.55 ± 2.05 vs. 1,015.15 ± 4.31 ms, p < 0.0001) was shorter than in the gene-inhibited group. Conclusion: Inhibition of sinus rhythm in the bradycardia cell model was recovered by treatment with ISO and MgSO4, demonstrating the potency of combination therapy in the treatment of bradycardia. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 15~24 | - |
dc.relation.isPartOf | CARDIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Bradycardia/drug therapy* | - |
dc.subject.MESH | Bradycardia/metabolism | - |
dc.subject.MESH | Cardiotonic Agents/pharmacology | - |
dc.subject.MESH | Cardiotonic Agents/therapeutic use* | - |
dc.subject.MESH | Cell Culture Techniques | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Drug Evaluation, Preclinical | - |
dc.subject.MESH | Isoproterenol/pharmacology | - |
dc.subject.MESH | Isoproterenol/therapeutic use* | - |
dc.subject.MESH | KCNQ1 Potassium Channel/genetics | - |
dc.subject.MESH | KCNQ1 Potassium Channel/metabolism* | - |
dc.subject.MESH | Magnesium Sulfate/pharmacology | - |
dc.subject.MESH | Magnesium Sulfate/therapeutic use* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Microelectrodes | - |
dc.subject.MESH | Myocytes, Cardiac/drug effects | - |
dc.subject.MESH | Myocytes, Cardiac/metabolism | - |
dc.subject.MESH | RNA Interference | - |
dc.subject.MESH | RNA, Small Interfering | - |
dc.title | Synergistic Restoring Effects of Isoproterenol and Magnesium on KCNQ1-Inhibited Bradycardia Cell Models Cultured in Microelectrode Array | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Kim S.K. | - |
dc.contributor.googleauthor | Pak H.-N. | - |
dc.contributor.googleauthor | Park Y. | - |
dc.identifier.doi | 10.1159/000356955 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01776 | - |
dc.relation.journalcode | J00457 | - |
dc.identifier.eissn | 1421-9751 | - |
dc.identifier.pmid | 24514589 | - |
dc.identifier.url | http://www.karger.com/Article/FullText/356955 | - |
dc.subject.keyword | Bradycardia | - |
dc.subject.keyword | HL-1 atrial myocytes | - |
dc.subject.keyword | KCNQ1 gene | - |
dc.subject.keyword | MgSO4 + ISO | - |
dc.subject.keyword | Potassium channel | - |
dc.contributor.alternativeName | Pak, Hui Nam | - |
dc.contributor.affiliatedAuthor | Pak, Hui Nam | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 128 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 15 | - |
dc.citation.endPage | 24 | - |
dc.identifier.bibliographicCitation | CARDIOLOGY, Vol.128(1) : 15-24, 2014 | - |
dc.identifier.rimsid | 38835 | - |
dc.type.rims | ART | - |
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