Cited 122 times in
Intestinal Brush Border Assembly Driven by Protocadherin-Based Intermicrovillar Adhesion
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 남기택 | - |
dc.date.accessioned | 2015-01-06T16:48:12Z | - |
dc.date.available | 2015-01-06T16:48:12Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/98799 | - |
dc.description.abstract | Transporting epithelial cells build apical microvilli to increase membrane surface area and enhance absorptive capacity. The intestinal brush border provides an elaborate example with tightly packed microvilli that function in nutrient absorption and host defense. Although the brush border is essential for physiological homeostasis, its assembly is poorly understood. We found that brush border assembly is driven by the formation of Ca2+-dependent adhesion links between adjacent microvilli. Intermicrovillar links are composed of protocadherin-24 and mucin-like protocadherin, which target to microvillar tips and interact to form a trans-heterophilic complex. The cytoplasmic domains of microvillar protocadherins interact with the scaffolding protein, harmonin, and myosin-7b, which promote localization to microvillar tips. Finally, a mouse model of Usher syndrome lacking harmonin exhibits microvillar protocadherin mislocalization and severe defects in brush border morphology. These data reveal an adhesion-based mechanism for brush border assembly and illuminate the basis of intestinal pathology in patients with Usher syndrome. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 433~446 | - |
dc.relation.isPartOf | CELL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | COS Cells | - |
dc.subject.MESH | Caco-2 Cells | - |
dc.subject.MESH | Cadherins/metabolism* | - |
dc.subject.MESH | Calcium/metabolism | - |
dc.subject.MESH | Carrier Proteins/genetics | - |
dc.subject.MESH | Carrier Proteins/metabolism | - |
dc.subject.MESH | Cercopithecus aethiops | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Enterocytes/cytology | - |
dc.subject.MESH | Enterocytes/metabolism* | - |
dc.subject.MESH | HEK293 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Knockout | - |
dc.subject.MESH | Microvilli/metabolism* | - |
dc.subject.MESH | Microvilli/ultrastructure | - |
dc.subject.MESH | Myosins/metabolism | - |
dc.subject.MESH | Usher Syndromes/pathology | - |
dc.title | Intestinal Brush Border Assembly Driven by Protocadherin-Based Intermicrovillar Adhesion | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Life Science (의생명과학부) | - |
dc.contributor.googleauthor | Scott W. Crawley | - |
dc.contributor.googleauthor | David A. Shifrin Jr. | - |
dc.contributor.googleauthor | Nathan E. Grega-Larson | - |
dc.contributor.googleauthor | Russell E. McConnell | - |
dc.contributor.googleauthor | Andrew E. Benesh | - |
dc.contributor.googleauthor | Suli Mao | - |
dc.contributor.googleauthor | Yuxi Zheng | - |
dc.contributor.googleauthor | Qing Yin Zheng | - |
dc.contributor.googleauthor | Ki Taek Nam | - |
dc.contributor.googleauthor | Bryan A. Millis | - |
dc.contributor.googleauthor | Bechara Kachar | - |
dc.contributor.googleauthor | Matthew J. Tyska | - |
dc.identifier.doi | 10.1016/j.cell.2014.01.067 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01243 | - |
dc.relation.journalcode | J00472 | - |
dc.identifier.eissn | 1097-4172 | - |
dc.identifier.pmid | 24725409 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0092867414002153 | - |
dc.contributor.alternativeName | Nam, Ki Taek | - |
dc.contributor.affiliatedAuthor | Nam, Ki Taek | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 157 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 433 | - |
dc.citation.endPage | 446 | - |
dc.identifier.bibliographicCitation | CELL, Vol.157(2) : 433-446, 2014 | - |
dc.identifier.rimsid | 39251 | - |
dc.type.rims | ART | - |
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