Cited 16 times in
Neuroprotective Effect of Melatonin against Kainic Acid-Induced Oxidative Injury in Hippocampal Slice Culture of Rats
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이배환 | - |
dc.date.accessioned | 2015-01-06T16:41:16Z | - |
dc.date.available | 2015-01-06T16:41:16Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/98572 | - |
dc.description.abstract | Endogenous melatonin is a known free radical scavenger that removes reactive oxygen species (ROS), thus, alleviating oxidative stress. The purpose of this study was to demonstrate its effect against kainic acid (KA)-induced oxidative stress in organotypic hippocampal slice cultures (OHSCs). To observe neuroprotective effects of melatonin, different concentrations (0.01, 0.1 and 1 mM) of melatonin were administrated after KA treatment for 18 h in OHSCs of rat pups. Dose-response studies showed that neuronal cell death was significantly reduced after 0.1 and 1 mΜ melatonin treatments based on propidium iodide (PI) uptake and cresyl violet staining. The dichlorofluorescein (DCF) fluorescence which indicates ROS formation decreased more in the melatonin-treated group than in the KA group. The expression of 5-lipoxigenase (5-LO) and caspase-3 were reduced in the melatonin-treated groups compared to the KA group. These results suggest that melatonin may be an effective agent against KA-induced oxidative stress in the OHSC model. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 5940~5951 | - |
dc.language | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.publisher | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antioxidants/pharmacology | - |
dc.subject.MESH | Arachidonate 5-Lipoxygenase/biosynthesis | - |
dc.subject.MESH | Caspase 3/biosynthesis | - |
dc.subject.MESH | Cell Death/drug effects* | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Hippocampus/cytology | - |
dc.subject.MESH | Hippocampus/pathology* | - |
dc.subject.MESH | Kainic Acid/toxicity* | - |
dc.subject.MESH | Melatonin/pharmacology* | - |
dc.subject.MESH | Neurons/cytology | - |
dc.subject.MESH | Neurons/metabolism | - |
dc.subject.MESH | Neuroprotective Agents/pharmacology* | - |
dc.subject.MESH | Oxidative Stress/drug effects | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Reactive Oxygen Species/metabolism | - |
dc.title | Neuroprotective Effect of Melatonin against Kainic Acid-Induced Oxidative Injury in Hippocampal Slice Culture of Rats | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학) | - |
dc.contributor.googleauthor | Hyung A Kim | - |
dc.contributor.googleauthor | Kyung Hee Lee | - |
dc.contributor.googleauthor | Bae Hwan Lee | - |
dc.identifier.doi | 10.3390/ijms15045940 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02791 | - |
dc.relation.journalcode | J01133 | - |
dc.identifier.eissn | 1422-0067 | - |
dc.identifier.pmid | 24722567 | - |
dc.subject.keyword | melatonin | - |
dc.subject.keyword | kainic acid | - |
dc.subject.keyword | organotypic hippocampal slice culture | - |
dc.subject.keyword | reactive oxygen species | - |
dc.subject.keyword | antioxidant | - |
dc.subject.keyword | neuroprotection | - |
dc.contributor.alternativeName | Lee, Bae Hwan | - |
dc.contributor.affiliatedAuthor | Lee, Bae Hwan | - |
dc.citation.volume | 15 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 5940 | - |
dc.citation.endPage | 5951 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.15(4) : 5940-5951, 2014 | - |
dc.identifier.rimsid | 38121 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.