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High-throughput, high-content screening for novel pigmentation regulators using a keratinocyte/melanocyte co-culture system

DC FieldValueLanguage
dc.contributor.author이주희-
dc.date.accessioned2015-01-06T16:38:27Z-
dc.date.available2015-01-06T16:38:27Z-
dc.date.issued2014-
dc.identifier.issn0906-6705-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/98482-
dc.description.abstractSkin pigmentation is a complex process including melanogenesis within melanocytes and melanin transfer to the keratinocytes. To develop a comprehensive screening method for novel pigmentation regulators, we used immortalized melanocytes and keratinocytes in co-culture to screen large numbers of compounds. High-throughput screening plates were subjected to digital automated microscopy to quantify the pigmentation via brightfield microscopy. Compounds with pigment suppression were secondarily tested for their effects on expression of microphthalmia transcription factor (MITF) and several pigment regulatory genes, and further validated in terms of non-toxicity to keratinocytes/melanocytes and dose-dependent activity. The results demonstrate a high-throughput, high-content screening approach, which is applicable to the analysis of large chemical libraries using a co-culture system. We identified candidate pigmentation inhibitors from 4000 screened compounds including zoxazolamine, 3-methoxycatechol and alpha-mangostin, which were also shown to modulate expression of MITF and several key pigmentation factors and are worthy of further evaluation for potential translation to clinical use.-
dc.description.statementOfResponsibilityopen-
dc.format.extent125~129-
dc.relation.isPartOfExperimental Dermatology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Line, Transformed-
dc.subject.MESHCoculture Techniques-
dc.subject.MESHEnzyme Inhibitors/isolation & purification*-
dc.subject.MESHEnzyme Inhibitors/pharmacology-
dc.subject.MESHGene Expression Regulation/drug effects*-
dc.subject.MESHHigh-Throughput Screening Assays*-
dc.subject.MESHIntramolecular Oxidoreductases/biosynthesis-
dc.subject.MESHIntramolecular Oxidoreductases/genetics-
dc.subject.MESHKeratinocytes/drug effects*-
dc.subject.MESHKeratinocytes/metabolism-
dc.subject.MESHMelanins/biosynthesis*-
dc.subject.MESHMelanins/genetics-
dc.subject.MESHMelanocytes/drug effects*-
dc.subject.MESHMelanocytes/metabolism-
dc.subject.MESHMelanoma, Experimental/pathology-
dc.subject.MESHMembrane Glycoproteins/biosynthesis-
dc.subject.MESHMembrane Glycoproteins/genetics-
dc.subject.MESHMice-
dc.subject.MESHMicrophthalmia-Associated Transcription Factor/biosynthesis-
dc.subject.MESHMicrophthalmia-Associated Transcription Factor/genetics-
dc.subject.MESHMonophenol Monooxygenase/biosynthesis-
dc.subject.MESHMonophenol Monooxygenase/genetics-
dc.subject.MESHOxidoreductases/biosynthesis-
dc.subject.MESHOxidoreductases/genetics-
dc.subject.MESHSkin Pigmentation/drug effects*-
dc.titleHigh-throughput, high-content screening for novel pigmentation regulators using a keratinocyte/melanocyte co-culture system-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Dermatology (피부과학)-
dc.contributor.googleauthorJu Hee Lee-
dc.contributor.googleauthorHongxiang Chen-
dc.contributor.googleauthorVihren Kolev-
dc.contributor.googleauthorKatherine H. Aull-
dc.contributor.googleauthorInhee Jung-
dc.contributor.googleauthorJun Wang-
dc.contributor.googleauthorShoko Miyamoto-
dc.contributor.googleauthorJunichi Hosoi-
dc.contributor.googleauthorAnna Mandinova-
dc.contributor.googleauthorDavid E. Fisher-
dc.identifier.doi10.1111/exd.12322-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03171-
dc.relation.journalcodeJ00866-
dc.identifier.pmid24438532-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/exd.12322/abstract-
dc.subject.keywordco-culture-
dc.subject.keywordhigh-content screening-
dc.subject.keywordkeratinocyte-
dc.subject.keywordmelanocyte-
dc.subject.keywordmicrophthalmia transcription factor-
dc.subject.keywordpigmentation-
dc.contributor.alternativeNameLee, Ju Hee-
dc.contributor.affiliatedAuthorLee, Ju Hee-
dc.rights.accessRightsfree-
dc.citation.volume23-
dc.citation.number2-
dc.citation.startPage125-
dc.citation.endPage129-
dc.identifier.bibliographicCitationExperimental Dermatology, Vol.23(2) : 125-129, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers

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