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Evaluation of a Rapid Membrane Enzyme Immunoassay for the Simultaneous Detection of Glutamate Dehydrogenase and Toxin for the Diagnosis of Clostridium difficile Infection

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dc.contributor.author김희정-
dc.contributor.author이경원-
dc.contributor.author정석훈-
dc.date.accessioned2015-01-06T16:36:32Z-
dc.date.available2015-01-06T16:36:32Z-
dc.date.issued2014-
dc.identifier.issn2234-3806-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/98423-
dc.description.abstractWe evaluated the new C. DIFF QUIK CHEK COMPLETE (CD COMPLETE; TechLab, USA), which is a rapid membrane enzyme immunoassay that uses a combination of glutamate dehydrogenase (GDH) antigen and toxin A and B detection. A total of 608 consecutive loose stool specimens collected from the patients with suspected Clostridium difficile infection (CDI) from August to December 2012 were subjected to the CD COMPLETE and VIDAS Clostridium difficile A & B (VIDAS CDAB; bioMérieux, France). Their performances were compared with a toxigenic culture as a reference. Stool specimens that were culture-negative and CD COMPLETE- or VIDAS CDAB-positive were analyzed by using an enrichment procedure. In comparison to the toxigenic cultures, sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV) were 63.6%, 98.0%, 76.1%, and 96.4%, respectively, for the CD COMPLETE-toxin and 75.5%, 97.4%, 72.5%, and 97.8%, respectively, for the VIDAS CDAB. In comparison to the enriched C. difficile cultures, the sensitivity, specificity, PPV, and NPV for the CD COMPLETE-GDH were 91.0%, 92.4%, 70.5%, and 98.1%, respectively. The CD COMPLETE is a reliable method for the diagnosis of CDI and provides greater sensitivity than toxin enzyme immunoassay alone. Furthermore, the CD COMPLETE-GDH has advantages over direct culture in detecting C. difficile.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1~5-
dc.relation.isPartOfANNALS OF LABORATORY MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBacterial Proteins/analysis*-
dc.subject.MESHBacterial Toxins/analysis*-
dc.subject.MESHClostridium Infections/diagnosis*-
dc.subject.MESHClostridium Infections/microbiology-
dc.subject.MESHClostridium difficile/enzymology-
dc.subject.MESHClostridium difficile/isolation & purification*-
dc.subject.MESHClostridium difficile/metabolism-
dc.subject.MESHEnterotoxins/analysis*-
dc.subject.MESHFeces/microbiology-
dc.subject.MESHGlutamate Dehydrogenase/analysis*-
dc.subject.MESHHumans-
dc.subject.MESHImmunoenzyme Techniques*-
dc.subject.MESHReagent Kits, Diagnostic-
dc.subject.MESHSensitivity and Specificity-
dc.titleEvaluation of a Rapid Membrane Enzyme Immunoassay for the Simultaneous Detection of Glutamate Dehydrogenase and Toxin for the Diagnosis of Clostridium difficile Infection-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorHeejung Kim-
dc.contributor.googleauthorWan Hee Kim-
dc.contributor.googleauthorMyungsook Kim-
dc.contributor.googleauthorSeok Hoon Jeong-
dc.contributor.googleauthorKyungwon Lee-
dc.identifier.doi10.3343/alm.2014.34.3.235-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02649-
dc.contributor.localIdA03619-
dc.contributor.localIdA01219-
dc.relation.journalcodeJ00164-
dc.identifier.eissn2234-3814-
dc.identifier.pmid24790912-
dc.subject.keywordClostridium difficile-
dc.subject.keywordCulture-
dc.subject.keywordEnzyme immunoassays-
dc.subject.keywordGlutamate dehydrogenase-
dc.subject.keywordToxin A-
dc.subject.keywordToxin B-
dc.contributor.alternativeNameKim, Hee Jung-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameJeong, Seok Hoon-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorJeong, Seok Hoon-
dc.contributor.affiliatedAuthorKim, Heejung-
dc.citation.volume34-
dc.citation.number3-
dc.citation.startPage1-
dc.citation.endPage5-
dc.identifier.bibliographicCitationANNALS OF LABORATORY MEDICINE, Vol.34(3) : 1-5, 2014-
dc.identifier.rimsid56592-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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