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Forensic interlaboratory evaluation of the ForFLUID kit for vaginal fluids identification

Authors
 Saverio Giampaoli  ;  Federica Alessandrini  ;  Andrea Berti  ;  Luigi Ripani  ;  Ajin Choi  ;  Roselien Crab  ;  Elisabetta De Vittori  ;  Balazs Egyed  ;  Cordula Haas  ;  Hwan Young Lee  ;  Marie Korabecná  ;  Fabrice Noel  ;  Daniele Podini  ;  Adriano Tagliabracci  ;  Alessio Valentinik  ;  Vincenzo Romano Spica 
Citation
 JOURNAL OF FORENSIC AND LEGAL MEDICINE, Vol.21 : 60-63, 2014 
Journal Title
JOURNAL OF FORENSIC AND LEGAL MEDICINE
ISSN
 1752-928X 
Issue Date
2014
MeSH
Adult ; Cervix Mucus/microbiology* ; DNA, Bacterial/genetics* ; Enterococcus faecalis/genetics ; Enterococcus faecalis/isolation & purification ; Female ; Forensic Medicine/standards ; Humans ; Laboratories/standards ; Lactobacillus/genetics ; Lactobacillus/isolation & purification ; Multiplex Polymerase Chain Reaction* ; Real-Time Polymerase Chain Reaction* ; Staphylococcus/genetics ; Staphylococcus/isolation & purification ; Streptococcus/genetics ; Streptococcus/isolation & purification ; Vagina/microbiology*
Keywords
Bacteria ; Forensic ; Interlaboratory evaluation ; Multiplex PCR ; Vaginal fluids ; mfDNA
Abstract
Identification of vaginal fluids is an important step in the process of sexual assaults confirmation. Advances in both microbiology and molecular biology defined technical approaches allowing the discrimination of body fluids. These protocols are based on the identification of specific bacterial communities by microfloraDNA (mfDNA) amplification. A multiplex real time-PCR assay (ForFLUID kit) has been developed for identifying biological fluids and for discrimination among vaginal, oral and fecal samples. In order to test its efficacy and reliability of the assay in the identification of vaginal fluids, an interlaboratory evaluation has been performed on homogeneous vaginal swabs. All the involved laboratories were able to correctly recognize all the vaginal swabs, and no false positives were identified when the assay was applied on non-vaginal samples. The assay represents an useful molecular tool that can be easily adopted by forensic geneticists involved in vaginal fluid identification.
Full Text
http://www.sciencedirect.com/science/article/pii/S1752928X13002904
DOI
10.1016/j.jflm.2013.10.016
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
Yonsei Authors
Lee, Hwan Young(이환영)
Choi, A Jin(최아진)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98090
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