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Distribution of MT1 Melatonin Receptor Promoter-Driven RFP Expression in the Brains of BAC C3H/HeN Transgenic Mice

Authors
 EB Adamah-Biassi  ;  Y Zhang  ;  H Jung  ;  S Vissapragada  ;  RJ Miller  ;  ML Dubocovich 
Citation
 JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, Vol.62(1) : 70-84, 2014 
Journal Title
 JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 
ISSN
 0022-1554 
Issue Date
2014
MeSH
Animals ; Brain/metabolism* ; Chromosomes, Artificial, Bacterial/genetics* ; Female ; Gene Expression ; Indoles/metabolism ; Luminescent Proteins/genetics* ; Male ; Mice ; Mice, Inbred C3H ; Mice, Transgenic ; Promoter Regions, Genetic/genetics* ; Receptor, Melatonin, MT1/genetics*
Keywords
C3H/HeN mice ; MT1 melatonin receptors ; RFP-MT1 promoter expression
Abstract
The pineal hormone melatonin activates two G-protein coupled receptors (MT1 and MT2) to regulate in part biological functions. The MT1 and MT2 melatonin receptors are heterogeneously distributed in the mammalian brain including humans. In the mouse, only a few reports have assessed the expression of the MT1 melatonin receptor expression using 2-iodomelatonin binding, in situ hybridization and/or polymerase chain reaction (PCR). Here, we described a transgenic mouse in which red fluorescence protein (RFP) is expressed under the control of the endogenous MT1 promoter, by inserting RFP cDNA at the start codon of MTNR1a gene within a bacterial artificial chromosome (BAC) and expressing this construct as a transgene. The expression of RFP in the brain of this mouse was examined either directly under a fluorescent microscope or immunohistochemically using an antibody against RFP (RFP-MT1). RFP-MT1 expression was observed in many brain regions including the subcommissural organ, parts of the ependyma lining the lateral and third ventricles, the aqueduct, the hippocampus, the cerebellum, the pars tuberalis, the habenula and the habenula commissure. This RFP-MT1 transgenic model provides a unique tool for studying the distribution of the MT1 receptor in the brain of mice, its cell-specific expression and its function in vivo.
Full Text
http://jhc.sagepub.com/content/62/1/70.long
DOI
10.1369/0022155413507453
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Jung, Ho Sung(정호성) ORCID logo https://orcid.org/0000-0002-5059-8050
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98078
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