554 761

Cited 0 times in

Mycophenolic acid가 올레산으로 유도한 흰쥐 혈관 평활근 세포의 증식에 미치는 영향

DC Field Value Language
dc.contributor.author김명수-
dc.contributor.author김유선-
dc.contributor.author주만기-
dc.contributor.author안형준-
dc.date.accessioned2014-12-21T17:30:48Z-
dc.date.available2014-12-21T17:30:48Z-
dc.date.issued2007-
dc.identifier.issn1226-0053-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/97732-
dc.description.abstractVascular smooth muscle cell (VSMC) proliferation plays an important role in the development and progression of chronic allograft vasculopathy. Mycophenolic acid (MPA) inhibits various mesenchymal cell proliferation, and reactive oxygen species (ROS) are involved in the anti-pro-liferative effect of MPA. In this study, we investigated the effects of MPA on oleic acid (OA)-induced VSMC proliferation and also the role of ROS in these processes. Methods: Primary cultured rat VSMCs from Sprague-Dawley were stimulated with OA 100μM. MPA 0.1∼10μM and N-acetylcystein (NAC) 5 mM were administered 1 hour before adding the OA. Cell proliferation was measured by Methylthiazoletetrazolium (MTT) assay, proliferating cell nuclear antigen (PCNA) expression by Western blot analysis, and dichlorofluorescein (DCF)-sensitive cellular ROS by flow cytometry. Results: OA at 100μM significantly increased MTT level by 1.6-fold as well as PCNA expression at 48 hours in rat VSMCs. OA also induced DCF-sensitive cellular ROS by 1.6-fold at 5 minutes and the increment of cellular ROS remained for up to 1 hour. MPA at above 1μM inhibited OA- induced VSMC proliferation and cellular ROS in a dosedependent manner. NAC 5 mM also inhibited OA-induced rat VSMC activation. Conclusion: These results suggest that MPA inhibits OA-induced VSMC proliferation partially through the inhibition of cellular ROS.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.publisher대한외과학회-
dc.relation.isPartOfJOURNAL OF THE KOREAN SURGICAL SOCIETY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleMycophenolic acid가 올레산으로 유도한 흰쥐 혈관 평활근 세포의 증식에 미치는 영향-
dc.title.alternativeEffects of Mycophenolic Acid on Oleic Acid- induced Rat Vascular Smooth Muscle Cell Proliferation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthor안형준-
dc.contributor.googleauthor박제현-
dc.contributor.googleauthor김유선-
dc.contributor.googleauthor송기호-
dc.contributor.googleauthor하헌주-
dc.contributor.googleauthor김명수-
dc.contributor.googleauthor주만기-
dc.contributor.googleauthor송재숙-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00785-
dc.contributor.localIdA02273-
dc.contributor.localIdA03949-
dc.contributor.localIdA00424-
dc.relation.journalcodeJ01893-
dc.contributor.alternativeNameKim, Myoung Soo-
dc.contributor.alternativeNameKim, Yu Seun-
dc.contributor.alternativeNameJoo, Man Ki-
dc.contributor.affiliatedAuthorKim, Yu Seun-
dc.contributor.affiliatedAuthorJoo, Man Ki-
dc.contributor.affiliatedAuthorKim, Myoung Soo-
dc.rights.accessRightsfree-
dc.citation.volume72-
dc.citation.number3-
dc.citation.startPage171-
dc.citation.endPage176-
dc.identifier.bibliographicCitationJOURNAL OF THE KOREAN SURGICAL SOCIETY, Vol.72(3) : 171-176, 2007-
dc.identifier.rimsid47671-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.