Cited 117 times in
Efficient Induction of Oligodendrocytes from Human Embryonic Stem Cells
DC Field | Value | Language |
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dc.contributor.author | 김동욱 | - |
dc.date.accessioned | 2014-12-21T17:19:13Z | - |
dc.date.available | 2014-12-21T17:19:13Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1066-5099 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/97366 | - |
dc.description.abstract | Oligodendrocytes form myelin sheaths around axons to support rapid nerve conduction in the central nervous system (CNS). Damage to myelin can cause severe CNS disorders. In this study, we attempted to devise a protocol for the induction of oligodendrocytes from human embryonic stem (ES) cells to treat demyelinated axons. Four days after embryoid body formation, human ES cells were differentiated into neural precursors through selection and expansion procedures. Neural precursors were then grown in the presence of epidermal growth factor and then platelet-derived growth factor to generate oligodendrocyte precursor cells. After withdrawal of the growth factors, the cells were treated with thyroid hormone to induce differentiation into oligodendrocytes. This method resulted in ∼81%–91% oligodendrocyte precursor cells and ∼81% oligodendrocytes among total cells. The ability of the oligodendrocyte precursors to myelinate axons has been verified by coculturing with rat hippocampal neurons, confirming their biological functionality. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 419~424 | - |
dc.relation.isPartOf | STEM CELLS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Efficient Induction of Oligodendrocytes from Human Embryonic Stem Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학) | - |
dc.contributor.googleauthor | Sang-Moon Kang | - |
dc.contributor.googleauthor | Myung Soo Cho | - |
dc.contributor.googleauthor | Dong-Wook Kim | - |
dc.contributor.googleauthor | Young Min Choi | - |
dc.contributor.googleauthor | Sun Kyung Oh | - |
dc.contributor.googleauthor | Chul Jong Yoon | - |
dc.contributor.googleauthor | Hyemyung Seo | - |
dc.identifier.doi | 10.1634/stemcells.2005-0482 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00406 | - |
dc.relation.journalcode | J02683 | - |
dc.identifier.eissn | 1549-4918 | - |
dc.contributor.alternativeName | Kim, Dong Wook | - |
dc.contributor.affiliatedAuthor | Kim, Dong Wook | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 25 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 419 | - |
dc.citation.endPage | 424 | - |
dc.identifier.bibliographicCitation | STEM CELLS, Vol.25(2) : 419-424, 2007 | - |
dc.identifier.rimsid | 50466 | - |
dc.type.rims | ART | - |
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