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Molecular cloning and the allergenic characterization of tropomyosin from Tyrophagus putrescentiae

Authors
 Kyoung Yong Jeong  ;  Haeseok Lee  ;  Tai-Soon Yong  ;  Chein-Soo Hong  ;  Han-Il Ree  ;  In-Yong Lee  ;  Jongweon Lee  ;  Jae Sik Lee 
Citation
 PROTEIN AND PEPTIDE LETTERS, Vol.14(5) : 431-436, 2007 
Journal Title
 PROTEIN AND PEPTIDE LETTERS 
ISSN
 0929-8665 
Issue Date
2007
Abstract
Storage mites have been recognized as a cause of asthma and rhinitis. Studies from several countries have shown that the IgE-mediated allergy to storage mites is of considerable importance, especially in rural populations. This study aimed to identify and characterize new allergens from Tyrophagus putrescentiae. A partial cDNA sequence encoding tropomyosin was isolated from the cDNA library by immunoscreening using anti-mouse IgG1 sera raised against T. putrescentiae whole body extract. The deduced amino acid sequence shares 64-94% identity with previously known allergenic tropomyosins. Its recombinant protein was produced by using a pET 28b expression system and purified by affinity chromatography using Ni-NTA agarose. The IgE reactivities of tropomyosins from T. putrescentiae and Dermatophagoides farinae were compared by enzyme linked immunosorbent assay (ELISA). Recombinant Tyr p 10 showed 12.5% (5/40) IgE-binding reactivity, whereas recombinant Der f 10 showed 25% (10/40) IgE-binding reactivity against the same sera from storage mite-sensitized and house dust mite-sensitized subjects. Both recombinant Tyr p 10 and Der f 10 showed little inhibition of IgE binding to T. putrescentiae crude extract by ELISA. Tropomyosin seems to contribute only a small portion of the cross-reactivity with house dust mites.
Full Text
http://www.eurekaselect.com/78274/article
DOI
10.2174/092986607780782777
Appears in Collections:
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Lee, In Yong(이인용)
Lee, Jae Sik(이재식)
Lee, Jong Weon(이종원)
Ree, Han Il(이한일)
Lee, Hae Seok(이해석)
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Hong, Chein Soo(홍천수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/97325
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