Cited 54 times in
Regulation of mucin gene expression by CREB via a nonclassical retinoic acid signaling pathway
DC Field | Value | Language |
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dc.contributor.author | 윤주헌 | - |
dc.date.accessioned | 2014-12-21T17:12:29Z | - |
dc.date.available | 2014-12-21T17:12:29Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/97149 | - |
dc.description.abstract | Vitamin A and its metabolite retinoic acid (RA) are essential elements for normal lung development and the differentiation of lung epithelial cells. We previously showed that RA rapidly activated cyclic AMP response element-binding protein (CREB) in a nonclassical manner in normal human tracheobronchial epithelial (NHTBE) cells. In the present study, we further demonstrated that this nonclassical signaling of RA on the activation of CREB plays a critical role in regulating the expression of airway epithelial cell differentiation markers, the MUC2, MUC5AC, and MUC5B genes. We found that RA rapidly activates the protein kinase Cα isozyme and transmits the activation signal to CREB via the Raf/MEK/extracellular signal-regulated kinase/p90 ribosomal S6 kinase (RSK) pathway. Activated RSK translocated from the cytoplasm to the nucleus, where it phosphorylates CREB. Activated CREB then binds to a cis-acting replication element motif on the promoter (at nucleotides [nt] −878 to −871) of the MUC5AC gene. The depletion of CREB using small interfering RNA abolished not only the RA-induced MUC5AC but also RA-induced MUC2 and MUC5B. Taken together, our findings demonstrate that CREB activation via this nonclassical RA signaling pathway may play an important role in regulating the expression of mucin genes and mediating the early biological effects of RA during normal mucous differentiation in NHTBE cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 6933~6947 | - |
dc.relation.isPartOf | MOLECULAR AND CELLULAR BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Regulation of mucin gene expression by CREB via a nonclassical retinoic acid signaling pathway | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학) | - |
dc.contributor.googleauthor | Seung-Wook Kim | - |
dc.contributor.googleauthor | Jeong Soo Hong | - |
dc.contributor.googleauthor | Ja Seok Koo | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.contributor.googleauthor | Wen-Cheng Chung | - |
dc.contributor.googleauthor | Seung-Hee Ryu | - |
dc.identifier.doi | 10.1128/MCB.02385-06 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02604 | - |
dc.relation.journalcode | J02243 | - |
dc.identifier.eissn | 1098-5549 | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 27 | - |
dc.citation.number | 19 | - |
dc.citation.startPage | 6933 | - |
dc.citation.endPage | 6947 | - |
dc.identifier.bibliographicCitation | MOLECULAR AND CELLULAR BIOLOGY, Vol.27(19) : 6933-6947, 2007 | - |
dc.identifier.rimsid | 55207 | - |
dc.type.rims | ART | - |
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