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Differential Expression of Nephrin According to Glomerular Size in Early Diabetic Kidney Disease

Authors
 Jin-Ju Kim  ;  Jin Ji Li  ;  Shin-Wook Kang  ;  Dae Suk Han  ;  Hyung Jong Kim  ;  Hoon Young Choi  ;  Seung Hyeok Han  ;  Tae-Hyun Yoo  ;  Dong-Ryeol Ryu  ;  Seung-Jae Kwak  ;  Dong-Sub Jung 
Citation
 JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, Vol.18(8) : 2303-2310, 2007 
Journal Title
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
ISSN
 1046-6673 
Issue Date
2007
MeSH
Albuminuria/pathology ; Albuminuria/physiopathology ; Animals ; Diabetic Nephropathies/pathology* ; Diabetic Nephropathies/physiopathology* ; Disease Progression ; Early Diagnosis ; Gene Expression Regulation ; Kidney Glomerulus/pathology* ; Kidney Glomerulus/physiology* ; Male ; Membrane Proteins/genetics* ; Microscopy, Electron ; Podocytes/pathology ; Podocytes/physiology ; Podocytes/ultrastructure ; RNA, Messenger/metabolism ; Rats ; Rats, Sprague-Dawley
Abstract
Diabetic nephropathy (DN) is clinically characterized by proteinuria. Many studies tried to demonstrate a relationship between proteinuria and changes in nephrin in various forms of glomerular diseases including DN, but the results are not consistent. Glomerular hypertrophy occurs in DN, yet hypertrophy does not develop in all glomeruli concurrently. For investigation of the differences in nephrin expression according to glomerular size, glomeruli were isolated from 10 control and 10 streptozotocin-induced diabetic rats at 6 wk after the induction of diabetes by a sieving technique using sieves with pore sizes of 250, 150, 125, and 75 microm. Glomeruli then were classified into large glomeruli (LG; on the 125-microm sieve) and small glomeruli (SG; on the 75-microm sieve) groups. Glomerular volumes were determined using an image analyzer, and mRNA and protein expression was determined by real-time PCR and Western blot, respectively. The mean volumes of diabetic LG (1.51 +/- 0.06 x 10(6) microm(3)) and control LG (1.37 +/- 0.05 x 10(6) microm(3)) were significantly higher than those of diabetic SG (0.94 +/- 0.03 x 10(6) microm(3)) and control SG (0.87 +/- 0.03 x 10(6) microm(3); P < 0.01). Nephrin mRNA expression was significantly reduced in the diabetic LG group compared with the diabetic SG and control glomeruli groups (P < 0.05). In contrast, nephrin mRNA expression was significantly higher in the diabetic SG group compared with the diabetic LG and control glomeruli groups (P < 0.05). Even after correction for 18s rRNA and Wilms' tumor-1 mRNA expression, the differences in nephrin mRNA expression remained significant. The expression of nephrin protein showed a similar pattern to the mRNA expression. In conclusion, these data suggest that the nephrin gene is differentially expressed according to glomerular size. Furthermore, more hypertrophied glomeruli with lesser nephrin expression may be responsible for albuminuria in the early stage of DN.
Files in This Item:
T200700041.pdf Download
DOI
10.1681/ASN.2006101145
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Shin Wook(강신욱) ORCID logo https://orcid.org/0000-0002-5677-4756
Han, Dae Suk(한대석)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/96544
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