Cited 67 times in
Detection of extended-spectrum β-lactamases by using boronic acid as an AmpC β-lactamase inhibitor in clinical isolates of Klebsiella spp. and Escherichia coli
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배일권 | - |
dc.contributor.author | 정석훈 | - |
dc.date.accessioned | 2014-12-21T16:44:27Z | - |
dc.date.available | 2014-12-21T16:44:27Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0095-1137 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/96264 | - |
dc.description.abstract | We evaluated highly sensitive methods using boronic acid (BA) to detect extended-spectrum beta-lactamase (ESBL) production. A total of 182 clinical isolates of Klebsiella spp. (n = 118) and Escherichia coli (n = 64) were analyzed: 62 harbored only ESBLs, 80 harbored both ESBLs and plasmid-mediated AmpC beta-lactamases (pAmpCs), and 40 harbored only pAmpCs. The CLSI confirmatory test detected all isolates that produce only ESBLs but detected 85% of isolates that produce both enzymes. When a >/=5-mm increase in the zone diameter of either the cefotaxime (CTX) or the ceftazidime (CAZ) disk in the presence of both clavulanic acid (CA) and BA was considered to be a positive result, the test detected all isolates that harbor ESBLs (+/- pAmpCs) but showed frequent false-positive results (50%) for isolates that produce only pAmpCs. Meanwhile, when a >/=3-mm increase in the zone diameter of either the CTX/BA or the CAZ/BA disk in the presence of CA was considered to be a positive result, the test also detected all isolates that harbor ESBLs (+/- pAmpCs) and showed less frequent false-positive results (5%) in isolates that produce only pAmpCs. The latter new interpretive guideline has enhanced detection of ESBLs in clinical isolates of Klebsiella spp. and Escherichia coli and allowed detection of an ESBL even when potentially masked by a pAmpC. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1180~1184 | - |
dc.relation.isPartOf | JOURNAL OF CLINICAL MICROBIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Bacterial Proteins/antagonists & inhibitors | - |
dc.subject.MESH | Boronic Acids/pharmacology* | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology | - |
dc.subject.MESH | Escherichia coli/drug effects* | - |
dc.subject.MESH | Escherichia coli/enzymology | - |
dc.subject.MESH | Escherichia coli Infections/microbiology | - |
dc.subject.MESH | False Positive Reactions | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Klebsiella/drug effects* | - |
dc.subject.MESH | Klebsiella/enzymology | - |
dc.subject.MESH | Klebsiella Infections/microbiology | - |
dc.subject.MESH | Microbial Sensitivity Tests/methods* | - |
dc.subject.MESH | Plasmids/genetics | - |
dc.subject.MESH | Predictive Value of Tests | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | beta-Lactam Resistance* | - |
dc.subject.MESH | beta-Lactamase Inhibitors | - |
dc.subject.MESH | beta-Lactamases/metabolism* | - |
dc.title | Detection of extended-spectrum β-lactamases by using boronic acid as an AmpC β-lactamase inhibitor in clinical isolates of Klebsiella spp. and Escherichia coli | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Wonkeun Song | - |
dc.contributor.googleauthor | Il Kwon Bae | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.contributor.googleauthor | Sang Hee Lee | - |
dc.contributor.googleauthor | Chae-Hoon Lee | - |
dc.contributor.googleauthor | You-Nae Lee | - |
dc.identifier.doi | 10.1128/JCM.02322-06 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01802 | - |
dc.contributor.localId | A03619 | - |
dc.relation.journalcode | J01325 | - |
dc.identifier.eissn | 1098-660X | - |
dc.identifier.pmid | 17301276 | - |
dc.contributor.alternativeName | Bae, Il Kwon | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | Bae, Il Kwon | - |
dc.contributor.affiliatedAuthor | Jeong, Seok Hoon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 45 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1180 | - |
dc.citation.endPage | 1184 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CLINICAL MICROBIOLOGY, Vol.45(4) : 1180-1184, 2007 | - |
dc.identifier.rimsid | 35019 | - |
dc.type.rims | ART | - |
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