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Cited 36 times in

Role of p53 in antioxidant defense of HPV-positive cervical carcinoma cells following H2O2 exposure

DC Field Value Language
dc.contributor.author김세헌-
dc.contributor.author조남훈-
dc.contributor.author조재호-
dc.contributor.author강숙희-
dc.date.accessioned2014-12-21T16:44:09Z-
dc.date.available2014-12-21T16:44:09Z-
dc.date.issued2007-
dc.identifier.issn0021-9533-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/96254-
dc.description.abstractIn HPV-positive cervical carcinoma cells, p53 protein is functionally antagonized by the E6 oncoprotein. We investigated a possible role of p53 in antioxidant defense of HPV-positive cervical cancer cell lines. We found that SiHa cells containing integrated HPV 16 had higher expression of p53 and exhibited the greatest resistant to H2O2-induced oxidative damage, compared with HeLa, CaSki and ME180 cell lines. Downregulation of p53 resulted in the inhibition of p53-regulated antioxidant enzymes and elevated intracellular ROS in SiHa cells. By contrast, the ROS level was not affected in HeLa, CaSki and ME180 cell lines after inhibition of the p53 protein. Under mild or severe H2O2-induced stress, p53-deficient SiHa cells exhibited much higher ROS levels than control SiHa cells. Furthermore, we analyzed cell viability and apoptosis after H2O2 treatment and found that p53 deficiency sensitized SiHa cells to H2O2 damage. Inhibition of p53 resulted in excessive oxidation of DNA; control SiHa cells exhibited a more rapid removal of 8-oxo-7,8-dihydro-2′-deoxyguanosine from DNA compared with p53-deficient SiHa cells exposed to the same level of H2O2 challenge. These data collectively show that endogenous p53 in SiHa cells has an antioxidant function and involves in the reinforcement of the antioxidant defense.-
dc.description.statementOfResponsibilityopen-
dc.format.extent2284~2294-
dc.relation.isPartOfJOURNAL OF CELL SCIENCE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAntioxidants/metabolism-
dc.subject.MESHDNA Damage/drug effects-
dc.subject.MESHDeoxyguanosine/analogs & derivatives-
dc.subject.MESHDeoxyguanosine/metabolism-
dc.subject.MESHDown-Regulation/drug effects-
dc.subject.MESHFemale-
dc.subject.MESHHeLa Cells-
dc.subject.MESHHuman papillomavirus 16*-
dc.subject.MESHHumans-
dc.subject.MESHHydrogen Peroxide/metabolism-
dc.subject.MESHHydrogen Peroxide/pharmacology*-
dc.subject.MESHOncogene Proteins, Viral/metabolism-
dc.subject.MESHOxidants/metabolism-
dc.subject.MESHOxidants/pharmacology*-
dc.subject.MESHOxidation-Reduction/drug effects-
dc.subject.MESHOxidative Stress/drug effects-
dc.subject.MESHPapillomavirus Infections/metabolism*-
dc.subject.MESHRepressor Proteins/metabolism-
dc.subject.MESHTumor Suppressor Protein p53/metabolism*-
dc.subject.MESHUterine Cervical Neoplasms/metabolism*-
dc.titleRole of p53 in antioxidant defense of HPV-positive cervical carcinoma cells following H2O2 exposure-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pathology (병리학)-
dc.contributor.googleauthorBoxiao Ding-
dc.contributor.googleauthorSung Gil Chi-
dc.contributor.googleauthorNam Hoon Cho-
dc.contributor.googleauthorDong Su Kim-
dc.contributor.googleauthorJae Ho Cho-
dc.contributor.googleauthorSuki Kang-
dc.contributor.googleauthorSe Heon Kim-
dc.identifier.doi10.1242/jcs.002345-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03901-
dc.contributor.localIdA00605-
dc.contributor.localIdA03812-
dc.contributor.localIdA00044-
dc.relation.journalcodeJ01301-
dc.identifier.eissn1477-9137-
dc.identifier.pmid17567683-
dc.subject.keywordp53-
dc.subject.keywordSiHa-
dc.subject.keywordH2O2-
dc.subject.keywordROS-
dc.subject.keywordOxidative stress-
dc.subject.keywordRNAinterference (RNAi)-
dc.contributor.alternativeNameKim, Se Heon-
dc.contributor.alternativeNameCho, Nam Hoon-
dc.contributor.alternativeNameCho, Jae Ho-
dc.contributor.affiliatedAuthorCho, Jae Ho-
dc.contributor.affiliatedAuthorKim, Se Heon-
dc.contributor.affiliatedAuthorCho, Nam Hoon-
dc.contributor.affiliatedAuthorKang, Suki-
dc.rights.accessRightsfree-
dc.citation.volume120-
dc.citation.numberpt13-
dc.citation.startPage2284-
dc.citation.endPage2294-
dc.identifier.bibliographicCitationJOURNAL OF CELL SCIENCE, Vol.120(pt13) : 2284-2294, 2007-
dc.identifier.rimsid35011-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers

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