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Evidence for two modes of development of acquired tumor necrosis factor-related apoptosis-inducing ligand resistance - Involvement of Bcl-xL

DC Field Value Language
dc.contributor.author송재진-
dc.date.accessioned2014-12-21T16:43:20Z-
dc.date.available2014-12-21T16:43:20Z-
dc.date.issued2007-
dc.identifier.issn0021-9258-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/96227-
dc.description.abstractPrevious studies have shown that repeated application of TRAIL induces acquired resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Using human prostate adenocarcinoma DU-145 and human pancreatic carcinoma MiaPaCa-2 cells as a model, we now demonstrate for the first time that two states of acquired TRAIL resistance can be developed after TRAIL treatment. Data from survival assay and Western blot analysis show that acquired TRAIL resistance was developed within 1 day and gradually decayed within 6 days after TRAIL treatment in both cell lines. After TRAIL treatment, the level of Bcl-xL increased and reached a maximum within 2 days and gradually decreased in both cell lines. Bcl-xL-mediated development of acquired TRAIL resistance was suppressed by knockdown of Bcl-xL expression. Protein interaction assay revealed that during the development of TRAIL resistance, Bcl-xL dissociated from Bad and then associated with Bax. Overexpression of mutant-type Bad (S136A), which prevents this dissociation, partially suppressed the development of acquired TRAIL resistance. Thus, our results suggest that (a) dissociation of Bad from Bcl-xL and (b) an increase in the intracellular level of Bcl-xL are responsible for development of acquired TRAIL resistance.-
dc.description.statementOfResponsibilityopen-
dc.format.extent319~328-
dc.relation.isPartOfJOURNAL OF BIOLOGICAL CHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHApoptosis-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Survival-
dc.subject.MESHGene Expression Regulation*-
dc.subject.MESHGossypol/pharmacology-
dc.subject.MESHHumans-
dc.subject.MESHMale-
dc.subject.MESHPancreatic Neoplasms/metabolism-
dc.subject.MESHProstatic Neoplasms/metabolism-
dc.subject.MESHProtein Binding-
dc.subject.MESHTNF-Related Apoptosis-Inducing Ligand/metabolism*-
dc.subject.MESHTransfection-
dc.subject.MESHbcl-Associated Death Protein/metabolism*-
dc.subject.MESHbcl-X Protein/metabolism*-
dc.titleEvidence for two modes of development of acquired tumor necrosis factor-related apoptosis-inducing ligand resistance - Involvement of Bcl-xL-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentDept. of Biomedical Sciences-
dc.contributor.googleauthorJae J. Song-
dc.contributor.googleauthorJee Young An-
dc.contributor.googleauthorYong J. Lee-
dc.contributor.googleauthorYong Tae Kwon-
dc.identifier.doi10.1074/jbc.M608065200-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02056-
dc.relation.journalcodeJ01258-
dc.identifier.eissn1083-351X-
dc.identifier.pmid17110373-
dc.contributor.alternativeNameSong, Jae Jin-
dc.contributor.affiliatedAuthorSong, Jae Jin-
dc.rights.accessRightsfree-
dc.citation.volume282-
dc.citation.number1-
dc.citation.startPage319-
dc.citation.endPage328-
dc.identifier.bibliographicCitationJOURNAL OF BIOLOGICAL CHEMISTRY, Vol.282(1) : 319-328, 2007-
dc.identifier.rimsid34992-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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