Cited 2 times in
Integrated in silico and biological validation of the blocking effect of C(o)t-1 DNA on microarray-CGH
DC Field | Value | Language |
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dc.contributor.author | 김기열 | - |
dc.contributor.author | 라선영 | - |
dc.contributor.author | 정현철 | - |
dc.contributor.author | 정희철 | - |
dc.date.accessioned | 2014-12-21T16:41:42Z | - |
dc.date.available | 2014-12-21T16:41:42Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/96174 | - |
dc.description.abstract | In array-CGH, various factors may act as variables influencing the result of experiments. Among them, Cot-1 DNA, which has been used as a repetitive sequence-blocking agent, may become an artifact-inducing factor in BAC array-CGH. To identify the effect of Cot-1 DNA on Microarray-CGH experiments, Cot-1 DNA was labeled directly and Microarray-CGH experiments were performed. The results confirmed that probes which hybridized more completely with Cot-1 DNA had a higher sequence similarity to the Alu element. Further, in the sex-mismatched Microarray-CGH experiments, the variation and intensity in the fluorescent signal were reduced in the high intensity probe group in which probes were better hybridized with Cot-1 DNA. Otherwise, those of the low intensity probe group showed no alterations regardless of Cot-1 DNA. These results confirmed by in silico methods that Cot-1 DNA could block repetitive sequences in gDNA and probes. In addition, it was confirmed biologically that the blocking effect of Cot-1 DNA could be presented via its repetitive sequences, especially Alu elements. Thus, in contrast to BAC-array CGH, the use of Cot-1 DNA is advantageous in controlling experimental variation in Microarray-CGH. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 901~908 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Algorithms | - |
dc.subject.MESH | Alu Elements | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | DNA/pharmacology* | - |
dc.subject.MESH | DNA Probes/chemistry | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Nucleic Acid Hybridization/drug effects* | - |
dc.subject.MESH | Nucleic Acid Hybridization/methods* | - |
dc.subject.MESH | Oligonucleotide Array Sequence Analysis/methods* | - |
dc.subject.MESH | Repetitive Sequences, Nucleic Acid | - |
dc.subject.MESH | Sequence Alignment | - |
dc.subject.MESH | Sequence Homology, Nucleic Acid | - |
dc.subject.MESH | Signal Processing, Computer-Assisted | - |
dc.title | Integrated in silico and biological validation of the blocking effect of C(o)t-1 DNA on microarray-CGH | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Oral Cancer Research Institute (구강종양연구소) | - |
dc.contributor.googleauthor | Seung-Hui Kang | - |
dc.contributor.googleauthor | Chan Hee Park | - |
dc.contributor.googleauthor | Hyun Cheol Chung | - |
dc.contributor.googleauthor | Sun Young Rha | - |
dc.contributor.googleauthor | Ki-Yeol Kim | - |
dc.contributor.googleauthor | Hei Cheul Jeung | - |
dc.identifier.doi | 10.3892/ijmm.19.6.901 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00337 | - |
dc.contributor.localId | A03773 | - |
dc.contributor.localId | A03794 | - |
dc.contributor.localId | A01316 | - |
dc.relation.journalcode | J01132 | - |
dc.identifier.eissn | 1791-244X | - |
dc.identifier.pmid | 17487422 | - |
dc.identifier.url | http://www.spandidos-publications.com/ijmm/19/6/901 | - |
dc.subject.keyword | Cot-1 DNA | - |
dc.subject.keyword | Microarray-CGH | - |
dc.subject.keyword | sequence similarity | - |
dc.subject.keyword | Alu repetitive elements | - |
dc.contributor.alternativeName | Kim, Ki Yeol | - |
dc.contributor.alternativeName | Rha, Sun Young | - |
dc.contributor.alternativeName | Chung, Hyun Cheol | - |
dc.contributor.alternativeName | Jeung, Hei Cheul | - |
dc.contributor.affiliatedAuthor | Kim, Ki Yeol | - |
dc.contributor.affiliatedAuthor | Chung, Hyun Cheol | - |
dc.contributor.affiliatedAuthor | Jeung, Hei Cheul | - |
dc.contributor.affiliatedAuthor | Rha, Sun Young | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 19 | - |
dc.citation.startPage | 901 | - |
dc.citation.endPage | 908 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, Vol.19 : 901-908, 2007 | - |
dc.identifier.rimsid | 34955 | - |
dc.type.rims | ART | - |
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