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Integrated in silico and biological validation of the blocking effect of C(o)t-1 DNA on microarray-CGH

DC Field Value Language
dc.contributor.author김기열-
dc.contributor.author라선영-
dc.contributor.author정현철-
dc.contributor.author정희철-
dc.date.accessioned2014-12-21T16:41:42Z-
dc.date.available2014-12-21T16:41:42Z-
dc.date.issued2007-
dc.identifier.issn1107-3756-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/96174-
dc.description.abstractIn array-CGH, various factors may act as variables influencing the result of experiments. Among them, Cot-1 DNA, which has been used as a repetitive sequence-blocking agent, may become an artifact-inducing factor in BAC array-CGH. To identify the effect of Cot-1 DNA on Microarray-CGH experiments, Cot-1 DNA was labeled directly and Microarray-CGH experiments were performed. The results confirmed that probes which hybridized more completely with Cot-1 DNA had a higher sequence similarity to the Alu element. Further, in the sex-mismatched Microarray-CGH experiments, the variation and intensity in the fluorescent signal were reduced in the high intensity probe group in which probes were better hybridized with Cot-1 DNA. Otherwise, those of the low intensity probe group showed no alterations regardless of Cot-1 DNA. These results confirmed by in silico methods that Cot-1 DNA could block repetitive sequences in gDNA and probes. In addition, it was confirmed biologically that the blocking effect of Cot-1 DNA could be presented via its repetitive sequences, especially Alu elements. Thus, in contrast to BAC-array CGH, the use of Cot-1 DNA is advantageous in controlling experimental variation in Microarray-CGH.-
dc.description.statementOfResponsibilityopen-
dc.format.extent901~908-
dc.relation.isPartOfINTERNATIONAL JOURNAL OF MOLECULAR MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAlgorithms-
dc.subject.MESHAlu Elements-
dc.subject.MESHBase Sequence-
dc.subject.MESHDNA/pharmacology*-
dc.subject.MESHDNA Probes/chemistry-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHMale-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHNucleic Acid Hybridization/drug effects*-
dc.subject.MESHNucleic Acid Hybridization/methods*-
dc.subject.MESHOligonucleotide Array Sequence Analysis/methods*-
dc.subject.MESHRepetitive Sequences, Nucleic Acid-
dc.subject.MESHSequence Alignment-
dc.subject.MESHSequence Homology, Nucleic Acid-
dc.subject.MESHSignal Processing, Computer-Assisted-
dc.titleIntegrated in silico and biological validation of the blocking effect of C(o)t-1 DNA on microarray-CGH-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentOral Cancer Research Institute (구강종양연구소)-
dc.contributor.googleauthorSeung-Hui Kang-
dc.contributor.googleauthorChan Hee Park-
dc.contributor.googleauthorHyun Cheol Chung-
dc.contributor.googleauthorSun Young Rha-
dc.contributor.googleauthorKi-Yeol Kim-
dc.contributor.googleauthorHei Cheul Jeung-
dc.identifier.doi10.3892/ijmm.19.6.901-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00337-
dc.contributor.localIdA03773-
dc.contributor.localIdA03794-
dc.contributor.localIdA01316-
dc.relation.journalcodeJ01132-
dc.identifier.eissn1791-244X-
dc.identifier.pmid17487422-
dc.identifier.urlhttp://www.spandidos-publications.com/ijmm/19/6/901-
dc.subject.keywordCot-1 DNA-
dc.subject.keywordMicroarray-CGH-
dc.subject.keywordsequence similarity-
dc.subject.keywordAlu repetitive elements-
dc.contributor.alternativeNameKim, Ki Yeol-
dc.contributor.alternativeNameRha, Sun Young-
dc.contributor.alternativeNameChung, Hyun Cheol-
dc.contributor.alternativeNameJeung, Hei Cheul-
dc.contributor.affiliatedAuthorKim, Ki Yeol-
dc.contributor.affiliatedAuthorChung, Hyun Cheol-
dc.contributor.affiliatedAuthorJeung, Hei Cheul-
dc.contributor.affiliatedAuthorRha, Sun Young-
dc.rights.accessRightsnot free-
dc.citation.volume19-
dc.citation.startPage901-
dc.citation.endPage908-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, Vol.19 : 901-908, 2007-
dc.identifier.rimsid34955-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers

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