6 332

Cited 30 times in

Y-STR analysis of degraded DNA using reduced-size amplicons

Authors
 Myung Jin Park  ;  Hwan Young Lee  ;  Kyoung-Jin Shin  ;  Seung-Chul Kang  ;  Ukhee Chung 
Citation
 INTERNATIONAL JOURNAL OF LEGAL MEDICINE, Vol.121(2) : 152-157, 2007 
Journal Title
 INTERNATIONAL JOURNAL OF LEGAL MEDICINE 
ISSN
 0937-9827 
Issue Date
2007
MeSH
Asian Continental Ancestry Group/genetics ; Chromosomes, Human, Y/genetics* ; DNA Fingerprinting/methods* ; DNA Primers ; Forensic Anthropology/methods* ; Genetic Markers ; Humans ; Korea ; Microsatellite Repeats/genetics* ; Polymerase Chain Reaction/methods* ; Sensitivity and Specificity
Keywords
Y chromosome ; STR ; AmpFlSTR Yfiler ; Miniplex ; Degraded DNA
Abstract
To increase the success rate of Y-STR genotyping for degraded DNA, we have developed two multiplex PCR sets for 21 Y-STR loci. Besides the 17 Y-STR loci of DYS19, DYS385, DYS389-I, DYS389-II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, and GATA H4.1 contained in a commercial Y-STR kit, AmpFlSTR® Yfiler™, the other four loci of DYS388, DYS446, DYS447, and DYS449 were also included in the multiplexes to increase the discrimination capacity. Among a total of 21 Y-STR loci, the primers for eight loci (DYS385, DYS390, DYS438, DYS446, DYS448, DYS449, and DYS635) were newly designed in the present study and nine loci (DYS385, DYS390, DYS391, DYS392, DYS438, DYS439, DYS448, and DYS635) have PCR amplicons smaller than those of the AmpFlSTR® Yfiler™ kit. A sensitivity test using serially diluted standard 9948 male DNA showed that all the values of Y-STR loci in the Y-miniplexes are reliable at template concentrations as low as 30 pg. We compared the effectiveness of the two multiplexes with the AmpFlSTR® Yfiler™ kit by using both enzymatically degraded DNA and 30 samples of 50-year-old skeletal remains. This comparison demonstrated that the new Y-miniplex sets can produce a better signal from degraded DNA than the AmpFlSTR® Yfiler™ kit.
Full Text
http://link.springer.com/article/10.1007%2Fs00414-006-0133-7
DOI
10.1007/s00414-006-0133-7
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
Yonsei Authors
Park, Myung Jin(박명진)
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
Lee, Hwan Young(이환영)
Chung, Uk Hee(정욱희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/96171
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse