Cited 41 times in
Calpain-dependent calpastatin cleavage regulates caspase-3 activation during apoptosis of Jurkat T cells induced by Entamoeba histolytica
DC Field | Value | Language |
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dc.contributor.author | 김경아 | - |
dc.contributor.author | 신명헌 | - |
dc.contributor.author | 이영아 | - |
dc.date.accessioned | 2014-12-21T16:40:14Z | - |
dc.date.available | 2014-12-21T16:40:14Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0020-7519 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/96127 | - |
dc.description.abstract | In this study, we investigated whether there is a signalling interaction between calpain and caspase-3 during apoptosis in Jurkat T cells by Entamoeba histolytica. When Jurkat cells were co-incubated with E. histolytica, phosphatidylserine externalisation and DNA fragmentation markedly increased compared with results for cells incubated with medium alone. In addition, E. histolytica strongly induced cleavage of caspases-3, -6, -7 and poly(ADP-ribose) polymerase. A rise in intracellular calcium levels and activation of calpain were seen in Jurkat cells after exposure to E. histolytica. Pretreatment of Jurkat cells with calpain inhibitor calpeptin effectively blocked E. histolytica-triggered cleavage of caspase-3 as well as calpain. In contrast, pan-caspase inhibitor did not affect E. histolytica-induced calpain activation. In addition, incubation with E. histolytica resulted in multiple fragmented bands of calpastatin, which is an endogenous inhibitor of calpain, in Jurkat T cells. Moreover, Entamoeba-induced calpastatin degradation was dramatically suppressed by pretreatment with calpeptin, but not by z-VAD-fmk. Entamoeba-induced DNA fragmentation was strongly retarded by z-VAD-fmk, but not calpeptin. Our results suggest that calpain-mediated calpastatin degradation plays a crucial role in regulation of caspase-3 activation during apoptosis of Jurkat T cells by E. histolytica. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1209~1219 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL FOR PARASITOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Chloromethyl Ketones/pharmacology | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | Calcium/metabolism | - |
dc.subject.MESH | Calcium-Binding Proteins/genetics* | - |
dc.subject.MESH | Calpain/metabolism | - |
dc.subject.MESH | Caspase 3/metabolism* | - |
dc.subject.MESH | Caspase Inhibitors | - |
dc.subject.MESH | DNA Cleavage* | - |
dc.subject.MESH | Dipeptides/pharmacology | - |
dc.subject.MESH | Entamoeba histolytica/metabolism* | - |
dc.subject.MESH | Entamoebiasis/enzymology* | - |
dc.subject.MESH | Entamoebiasis/immunology | - |
dc.subject.MESH | Entamoebiasis/pathology | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Host-Parasite Interactions | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Jurkat Cells | - |
dc.subject.MESH | Parasitology/methods | - |
dc.subject.MESH | T-Lymphocytes/enzymology* | - |
dc.subject.MESH | T-Lymphocytes/pathology | - |
dc.title | Calpain-dependent calpastatin cleavage regulates caspase-3 activation during apoptosis of Jurkat T cells induced by Entamoeba histolytica | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Kyeong Ah Kim | - |
dc.contributor.googleauthor | Young Ah Lee | - |
dc.contributor.googleauthor | Myeong Heon Shin | - |
dc.identifier.doi | 10.1016/j.ijpara.2007.03.011 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02099 | - |
dc.contributor.localId | A02959 | - |
dc.contributor.localId | A00300 | - |
dc.relation.journalcode | J01082 | - |
dc.identifier.eissn | 1879-0135 | - |
dc.identifier.pmid | 17498717 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0020751907001063 | - |
dc.contributor.alternativeName | Kim, Kyung Ah | - |
dc.contributor.alternativeName | Shin, Myeong Heon | - |
dc.contributor.alternativeName | Lee, Young Ah | - |
dc.contributor.affiliatedAuthor | Shin, Myeong Heon | - |
dc.contributor.affiliatedAuthor | Lee, Young Ah | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Ah | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 37 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1209 | - |
dc.citation.endPage | 1219 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL FOR PARASITOLOGY, Vol.37(11) : 1209-1219, 2007 | - |
dc.identifier.rimsid | 35449 | - |
dc.type.rims | ART | - |
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